Postnatal development of GABA‐ergic neurons in the rabbit retina
- 1 September 1980
- journal article
- Published by Wiley in Journal of Comparative Neurology
- Vol. 193 (1), 89-102
- https://doi.org/10.1002/cne.901930107
Abstract
Uptake, synthesis, storage, and release of γ‐aminobutyric acid (GABA) are some of the characteristic properties of GABA‐ergic neurons. In the present study, we have used these properties as physiological probes to follow the emergence and maturation of GABA‐ergic neurons during postnatal development of the rabbit retina. There is autoradiographic, immunocytochemical, and pharmacological evidence that some amacrine cells and certain neurons in the ganglion cell layer probably use GABA as the neurotransmitter. These neurons take up GABA, contain the GABA‐synthesizing enzyme L‐glutamic acid decarboxylase (GAD, EC 4.1.1.15), and release the accumulated GABA by a Ca++‐dependent mechanism when depolorized with high extracellular K+ concentration. In this study, we show that certain neurons in the newborn retina already possess a specific mechanism for GABA uptake. The positions and numbers of these cells in the developing retina suggest that they will become GABA‐ergic neurons in the adultretina. These putative GABA‐ergic neurons are, however, probably immature an birth because newborn retinas contain only low levels of GABA and GAD. Additionally, there is relatively little K+‐stimulated, Ca++‐dependent release of (3H)‐GABA from the newborn retinas. GABA concentrations and GAD activities in developing retinas increase steadily postnatally, reaching about 80% of the adult levels by day 9. The activities of the GABA‐degrading enzyme, GABA‐glutamate transaminase (GABA‐T, EC 2.6.1.19), follow a similar pattern of maturation during retinal development. K+stimulated GABA release, however, remains low until about day 6, and then increases dramatically from 20% to 85% of the adult level over the next 3 days. Taken together, our results indicate that in the rabbit retina, the commitment by certain neurons to use GABA as the transmitter is made prenatally. These neurons are immature at birth but are biochemically, physiologically, and probably functionally mature by about 9 days after birth.Keywords
This publication has 29 references indexed in Scilit:
- Immunocytochemical localization of GABA neurons in the rabbit and frog retinaBrain Research Bulletin, 1980
- The gamma-aminobutyric acid system in rabbit retina: localization by immunocytochemistry and autoradiography.Proceedings of the National Academy of Sciences of the United States of America, 1979
- Effects of picrotoxin and strychnine on rabbit retinal ganglion cells: changes in centre surround receptive fields.The Journal of Physiology, 1978
- Effects of picrotoxin and strychnine on rabbit retinal ganglion cells: lateral interactions for cells with more complex receptive fields.The Journal of Physiology, 1978
- Glial and neuronal uptake of GABA, glutamic acid, glutamine and glutathione in the rabbit retinaExperimental Eye Research, 1977
- The inner plexiform layer of the vertebrate retina: A quantitative and comparative electron microscopic analysisJournal of Comparative Neurology, 1970
- Autoradiographic identification of rabbit retinal neurons that take up GABACellular and Molecular Life Sciences, 1970
- Neurotransmission in central nervous tissue: a study of isolated rabbit retina.Journal of Neurophysiology, 1969
- Synaptic organization of the frog retina: an electron microscopic analysis comparing the retinas of frogs and primatesProceedings of the Royal Society of London. B. Biological Sciences, 1968
- Retinal ganglion cells responding selectively to direction and speed of image motion in the rabbitThe Journal of Physiology, 1964