REUTILIZATION OF LYMPHOCYTE DNA BY CELLS OF INTESTINAL CRYPTS AND REGENERATING LIVER
Open Access
- 1 September 1963
- journal article
- research article
- Published by Rockefeller University Press in The Journal of cell biology
- Vol. 18 (3), 515-523
- https://doi.org/10.1083/jcb.18.3.515
Abstract
Lymphoid cells from mice injected 54 hours and 30 hours earlier with 3H-thymidine were washed and transfused into isogenic recipients at 29 to 30 hours after partial hepatectomy. The recipients were killed 28 to 30 hours later, and liver, intestine, Peyer''s patch, spleen, and the transfused cells were examined in autoradiographs exposed 6 months. Approximately 80 per cent of the labeled transfused cells were classed as lymphocytes. The labeled DNA contained in the transfused cells was partitioned to about 14 times as many recipient liver and intestinal cells, appearing in 72 to 78 per cent of hepatocyte nuclei, in 30 to 35 per cent of liver reticuloendothelial nuclei, and in 90 to 95 per cent of intestinal crypt nuclei. The label was not comparably widespread in the lymphoid organs, but was limited to a few intensely labeled lymphocytes and a somewhat larger number of very weakly labeled cells. When heat-killed cells rather than living cells were transfused, intensely labeled lymphocytes were absent from the lymphoid organs, but the labeling of cells in the recipients was otherwise identical. The results suggest that (a) reutilized DNA is derived from dead cells, (b) reutilized DNA is mainly degraded to nucleosides and nucleotides, the usual immediate de novo DNA precursors, before reincorporation into DNA, and (c) DNA reutilization may occur in the lymphoid organs, but on a less active scale than in intestine or regenerating liver.Keywords
This publication has 22 references indexed in Scilit:
- THE IN VIVO REUTILIZATION OF LYMPHOCYTIC AND SARCOMA DNA BY CELLS GROWING IN THE PERITONEAL CAVITYThe Journal of cell biology, 1962
- Comparative Study of the Kinetics of Cellular Proliferation of Normal and Tumorous Tissues With the Use of Tritiated Thymidine. I. Dilution of the Label and Migration of Labeled Cells2JNCI Journal of the National Cancer Institute, 1962
- AUTORADIOGRAPHIC ANALYSIS OF THE ACCUMULATION OF LYMPHOCYTES IN WOUNDSActa Pathologica Microbiologica Scandinavica, 1961
- Changes in effective enzyme concentration in the growing rat liver. II. Liver regeneration after partial hepatectomy.1961
- The Effects of Thymidine Administration on Thymidylate Kinase Activity and on DNA Synthesis in Mammalian TissuesCold Spring Harbor Symposia on Quantitative Biology, 1961
- Mechanism of growth. I. Interrelation between deoxyribonuclease and deoxyribonucleic acid synthesis in nonmalignant growth.1959
- REGULATION OF THE LEUKOCYTE LEVEL*Annals of the New York Academy of Sciences, 1959
- Effect of X-Radiation on DNA Metabolism in Various Tissues of the Rat: I. Incorporation of C 14 -Thymidine into DNA during the First 24 Hours PostirradiationRadiation Research, 1959
- DEOXYRIBOSYL COMPOUNDS IN ANIMAL TISSUESJournal of Biological Chemistry, 1958
- The stripping-film technique of autoradiographyThe International Journal of Applied Radiation and Isotopes, 1956