Differential modulation of human β-defensins expression in human gingival epithelia by Porphyromonas gingivalis lipopolysaccharide with tetra- and penta-acylated lipid A structures
- 12 August 2009
- journal article
- other
- Published by SAGE Publications in Innate Immunity
- Vol. 15 (6), 325-335
- https://doi.org/10.1177/1753425909104899
Abstract
Porphyromonas gingivalis lipopolysaccharide (LPS) is a crucial virulence factor strongly involved in the development of chronic periodontitis. It displays a significant amount of lipid A structural heterogeneity, containing both tetra- (LPS1435/1449) and penta-acylated (LPS1690) lipid A structures with opposing effects on E-selectin expression in human endothelial cells. Little is known about how these two isoforms of P. gingivalis LPS could differentially affect host innate immune responses in human gingival epithelia. The present study compares the modulatory effects of P. gingivalis LPS1435/1449and LPS1690on the expression of human β-defensins (hBDs) in the reconstituted human gingival epithelium, and examines the involvements of a panel of pattern recognition receptors in the modulatory effects concerned. It is shown that hBD-1, hBD-2 and hBD-3 mRNAs are significantly up-regulated by P. gingivalis LPS1690, but down-regulated by P. gingivalis LPS1435/1449. Toll-like receptor (TLR) 2 and CD14 mRNAs are also differentially regulated, and the modulation of hBD-2 expression may be through the co-operation of both TLR2 and TLR4. This study suggests that P. gingivalis LPS with different lipid A structures could differentially modulate host innate immune responses in human gingival epithelia, which may be a hitherto undescribed novel pathogenic mechanism of P. gingivalis in periodontal pathogenesis.Keywords
This publication has 34 references indexed in Scilit:
- Interleukin-1β Modulates Proinflammatory Cytokine Production in Human Epithelial CellsInfection and Immunity, 2008
- TLR4 and S1P receptors cooperate to enhance inflammatory cytokine production in human gingival epithelial cellsEuropean Journal of Immunology, 2008
- Antimicrobial peptide modulation in a differentiated reconstructed gingival epitheliumCell and tissue research, 2007
- Hemin-Dependent Modulation of the Lipid A Structure of Porphyromonas gingivalis LipopolysaccharideInfection and Immunity, 2006
- Porphyromonas gingivalis lipopolysaccharide lipid A heterogeneity: differential activities of tetra- and penta-acylated lipid A structures on E-selectin expression and TLR4 recognitionCellular Microbiology, 2006
- Occurrence of Porphyromonas gingivalis and Tannerella forsythensis in Periodontally Diseased and Healthy SubjectsThe Journal of Periodontology, 2004
- Expression of human β‐defensins‐1 and ‐2 peptides in unresolved chronic periodontitisJournal of Periodontal Research, 2004
- Identification of a novel, multifunctional *-defensin (human *-defensin 3) with specific antimicrobial activityCell and tissue research, 2001
- Inducible Expression of Human β-Defensin 2 byFusobacterium nucleatumin Oral Epithelial Cells: Multiple Signaling Pathways and Role of Commensal Bacteria in Innate Immunity and the Epithelial BarrierInfection and Immunity, 2000
- Transcriptional Regulation of β-Defensin Gene Expression in Tracheal Epithelial CellsInfection and Immunity, 2000