Molecular identification and phylogeny of parasitic wasp species (Hymenoptera: Trichogrammatidae) by mitochondrial DNA RFLP and RAPD markers
- 1 November 1994
- journal article
- research article
- Published by Wiley in Insect Molecular Biology
- Vol. 3 (4), 229-237
- https://doi.org/10.1111/j.1365-2583.1994.tb00171.x
Abstract
Mitochondrial DNA (mtDNA) restriction fragment length polymorphism (RFLP) and random amplified polymorphic DNA (RAPD) were tested for their ability to discriminate between seven species of minute parasitic wasps belonging to the genus Trichogramma. They proved to be reliable species-diagnostic molecular markers. Pairwise comparisons of the mtDNA restriction maps revealed considerable differentiation among the seven species. Percentage of common restriction sites ranged from 30% to 83%. Phylogenetic analyses performed either on the mtDNA nucleotide distance matrix or on the matrix of the restriction site-state generated a tree congruent with those based on allozymes and morphology. RAPD procedures also revealed species-specific banding patterns and seem promising for a rapid and easy identification of Trichogramma species. Moreover, for some Trichogramma species, RAPD banding patterns might be informative of the phylogenetic relatedness.Keywords
This publication has 40 references indexed in Scilit:
- Characterization of differences between whiteflies using RAPD-PCRInsect Molecular Biology, 1993
- Mitochondrial and ribosomal DNA variation among members of the Anopheles quadrimaculatus (Diptera: Culicidae) species complexGenome, 1992
- Intra- and inter-nest variation in mitochondrial DNA in the polygynous antLeptothorax acervorum (Hymenoptera; Formicidae)Insectes Sociaux, 1992
- Variation in Mitochondrial DNA of Aedes Species (Diptera: Culicidae)Evolution, 1991
- Mitochondrial DNA variation in social wasps (Hymenoptera, Vespidae)Cellular and Molecular Life Sciences, 1990
- Polymorphisms in mitochondrial DNA of european and Africanized honeybees (Apis mellifera)Cellular and Molecular Life Sciences, 1988
- Mitochondrial DNA evolution in themelanogaster species subgroup ofDrosophilaJournal of Molecular Evolution, 1986
- Mathematical model for studying genetic variation in terms of restriction endonucleases.Proceedings of the National Academy of Sciences, 1979
- Biosystematics of Trichogramma and Trichogrammatoidea SpeciesAnnual Review of Entomology, 1977