Cytochemical localization of ATPase plasma membrane and acid phosphatase by cerium-based method in a salt-adapted cell line ofPisum sativum

Abstract

In order to study a possible application of cerium-based techniques in plant cells, ATPase and acid phosphatase activities have been compared in two cell lines of Pisum sativum calli, one sensitive to NaCI and the other selected to be grown under salinity (85 mM NaCI). ATPase activity was unchanged and localized in the plasma membrane of both cell lines. Acid phosphatase activity was significantly increased in the salt-selected line and localized in the cell walls, Golgi complex, multivesicular bodies and vacuoles. These results indicated a possible involvement of both activities in the maintenance of cell growth in the selected line under saline conditions.