A simple method for the quantitative analysis of urinary delta-aminol evulinic acid to evaluate lead absorption

Abstract

Wada, O., Toyokawa, K., Urata, G., Yano, Y., and Nakao, K. (1969).Brit. J. industr. Med.,26, 240-243. A simple method for the quantitative analysis of urinary delta-aminolevulinic acid to evaluate lead absorption. A procedure is given for the rapid, quantitative determination of urinary delta-aminolevulinic acid (ALA). Interfering substances are removed by n-butanol extraction. After pyrrole formation with ethyl acetoacetate, Ehrlich's reagent is added to produce the chromophore, which is then extracted with chloroform and measured spectrophotometrically or by comparison of the depth of colour with standard colour solutions. The recoveries were about 91% and the results agreed well with those obtained using ion-exchange column chromatography (r=0·985). This assay is simple, dependable, and suitable for large-scale screening of industrial workers exposed to lead poisoning, because the critical level of urinary ALA (20 mg./l. urine), which indicates dangerous lead absorption, gives a convenient absorbance.