Mycobacteria are hidden endophytes in the shoots of rock plant [Pogonatherum paniceum (Lam.) Hack.] (Poaceae)
- 16 July 2010
- journal article
- Published by Wiley in Environmental Microbiology Reports
- Vol. 2 (4), 619-624
- https://doi.org/10.1111/j.1758-2229.2010.00197.x
Abstract
A mycobacterium was isolated from micropropagated Pogonatherum paniceum and identified as a close relative of Mycobacterium cookii. The endophyte diversity in the shoots of potted and micropropagated P. paniceum plants was studied by culture-independent techniques. Group- and strain-specific PCR demonstrated that the P. paniceum plants harboured the isolated Mycobacterium strain as a minority. Altogether 101 clones of the PCR products were sequenced. The shoots of potted P. paniceum plants harboured unculturable endophytes in the families Phyllobacteriaceae, Hyphomicrobiaceae, Sphingobacteriaceae, Enterobacteriaceae, Alcaligenaceae and Mycobacteriaceae. Among the unculturable Mycobacteriaceae strains related to Mycobacterium chubuense, M. poriferae, M. obuense, M. fortuitum, M. neoaurum, M. diernhoferi, M. intracellulare and M. cookii were identified. Three unique sequences that clustered with M. llatzarense and M. mucogenicum were identified in micropropagated plants. According to the results, the shoots and micropropagated tissues of rock plant are inhabited by mycobacteria, which should stimulate further studies on the diversity of unculturable mycobacteria in edible crop plants.Keywords
This publication has 23 references indexed in Scilit:
- Methylobacteriumsp. resides in unculturable state in potato tissuesin vitroand becomes culturable after induction byPseudomonas fluorescensIMGB163Journal of Applied Microbiology, 2009
- Ubiquitous presence of normally non-culturable endophytic bacteria in field shoot-tips of banana and their gradual activation to quiescent cultivable form in tissue culturesPlant Cell, Tissue and Organ Culture (PCTOC), 2008
- MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) Software Version 4.0Molecular Biology and Evolution, 2007
- Culturable Endophytic Bacterial Flora of the Maturing Leaves and Roots of Rice Plants (Oryza sativa) Cultivated in a Paddy FieldMicrobes and Environments, 2007
- 16S rRNA targeted sandwich hybridization method for direct quantification of mycobacteria in soilsJournal of Microbiological Methods, 2006
- Bacterial Endophytes and Their Interactions with HostsMolecular Plant-Microbe Interactions®, 2006
- Analysis of the Endophytic Actinobacterial Population in the Roots of Wheat ( Triticum aestivum L.) by Terminal Restriction Fragment Length Polymorphism and Sequencing of 16S rRNA ClonesApplied and Environmental Microbiology, 2004
- Use of the Polymerase Chain Reaction and DNA Sequencing for Detection of Bartonella quintana in the Aortic Valve of a Patient with Culture-Negative Infective EndocarditisClinical Infectious Diseases, 1995
- Basic local alignment search toolJournal of Molecular Biology, 1990
- A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue CulturesPhysiologia Plantarum, 1962