Inhibitory effects ofAster spathulifoliusextract on adipogenesis and lipid accumulation in 3T3-L1 preadipocytes
Open Access
- 16 October 2015
- journal article
- Published by Oxford University Press (OUP) in Journal of Pharmacy and Pharmacology
- Vol. 68 (1), 107-118
- https://doi.org/10.1111/jphp.12485
Abstract
Objectives: A ster spathulifolius Maxim (AS), known for its anti-viral and anti-allergic activity, is also known to reduce body weight gain in high fat diet-induced obese rats. But its molecular mechanism of the anti-obesity effects is still unclear. So, we investigated the inhibitory effect of AS extract (ASE) on adipogenesis and lipid accumulation to determine the underlying cellular molecular mechanism.Methods: To perform this study, the contents of intracellular triglyceride were analysed. Real-time polymerase chain reaction and Western blotting were carried out to investigate the expression of adipogenic transcriptional factors.Key findings: ASE showed the suppression of adipogenic differentiation and the considerable reduction of the lipid accumulation in 3T3-L1 cells. Especially, ASE inhibited the early stage of differentiation via the downregulation of C/EBP-β and C/EBP-δ, which are early adipogenic factors. Major adipogenic factors, such as PPAR-γ and C/EBP-α, were also subsequently inhibited. These findings were supported by Oil Red O staining and intracellular triglyceride levels. A molecular mechanism liking the effect of ASE was identified through the activation of AMPKα pathway. ASE increased protein levels of phosphorylated AMPKα and phosphorylated ACC.Conclusions: ASE showed anti-adipogenic and anti-lipogenic effects through the regulation of adipogenic factors and AMPKα pathway.Keywords
Funding Information
- Kyung Hee University (KHU-20050434)
- Ministry of Trade, Industry & Energy (10045257)
This publication has 63 references indexed in Scilit:
- Reduction of Adipogenesis and Lipid Accumulation by Taraxacum officinale (Dandelion) Extracts in 3T3L1 Adipocytes: An in vitro StudyPhytotherapy Research, 2013
- Anti-Obesity Effect of Artemisia capillaris Extracts in High-Fat Diet-Induced Obese RatsMolecules, 2013
- Chlorogenic Acid Stimulates Glucose Transport in Skeletal Muscle via AMPK Activation: A Contributor to the Beneficial Effects of Coffee on DiabetesPLOS ONE, 2012
- Global nutrition transition and the pandemic of obesity in developing countriesNutrition Reviews, 2012
- Dietary phytochemicals and their potential effects on obesity: A reviewPharmacological Research, 2011
- AMPK Phosphorylates and Inhibits SREBP Activity to Attenuate Hepatic Steatosis and Atherosclerosis in Diet-Induced Insulin-Resistant MiceCell Metabolism, 2011
- Persicaria hydropiper (L.) Spach and its Flavonoid Components, Isoquercitrin and Isorhamnetin, Activate the Wnt/β‐catenin Pathway and Inhibit Adipocyte Differentiation of 3T3‐L1 CellsPhytotherapy Research, 2011
- Metabolic effects of obesity: A reviewWorld Journal of Diabetes, 2010
- Adipocyte differentiation from the inside outNature Reviews Molecular Cell Biology, 2006
- Sirt1 promotes fat mobilization in white adipocytes by repressing PPAR-γNature, 2004