New PCR method to differentiate species in theAspergillus nigeraggregate
Open Access
- 1 November 1999
- journal article
- Published by Oxford University Press (OUP) in FEMS Microbiology Letters
- Vol. 180 (2), 191-196
- https://doi.org/10.1111/j.1574-6968.1999.tb08795.x
Abstract
The DNA that encodes the 5.8S gene of the ribosomal RNA and the two intergenic spacers ITS1 and ITS2 of the two proposed type strains of the Aspergillus niger aggregate (A. niger and Aspergillus tubingensis) have been sequenced. By comparison of sequences we have found that both species could be differentiated by RsaI digestion of the PCR products of the mentioned regions. This method could be a useful tool in the identification of strains of the A. niger aggregate, especially in studies that involve a large number of isolates.Keywords
This publication has 9 references indexed in Scilit:
- Evaluation of RFLP analysis of the classification of selected black aspergilliMycological Research, 1997
- Sequence or structure?: A short review on the application of nucleic acid sequence information to fungal taxonomyMycologist, 1995
- CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choiceNucleic Acids Research, 1994
- Mitochondrial DNA restriction fragment length polymorphisms in field isolates of the Aspergillus niger aggregateCanadian Journal of Microbiology, 1994
- The Genus AspergillusPublished by Springer Science and Business Media LLC ,1994
- Restriction fragment length polymorphisms in the mitochondrial DNAs of the Aspergillus niger aggregateMycological Research, 1993
- Genetic variability and relatedness in the complex group of black Aspergilli based on random amplification of polymorphic DNACurrent Genetics, 1993
- The use of RFLP analysis in classification of the black Aspergilli: reinterpretation of the Aspergillus niger aggregateCurrent Genetics, 1991
- AMPLIFICATION AND DIRECT SEQUENCING OF FUNGAL RIBOSOMAL RNA GENES FOR PHYLOGENETICSPublished by Elsevier BV ,1990