In Vitro and in Vivo Studies of FePt Nanoparticles for Dual Modal CT/MRI Molecular Imaging
- 24 June 2010
- journal article
- research article
- Published by American Chemical Society (ACS) in Journal of the American Chemical Society
- Vol. 132 (38), 13270-13278
- https://doi.org/10.1021/ja1035013
Abstract
The water-solvable FePt nanoparticles of 3, 6, and 12 nm in diameter (3 nm-, 6 nm-, and 12 nm-FePt) were synthesized and applied as a dual modality contrast agent for CT/MRI molecular imaging. These nanoparticles present excellent biocompatibility and hemocompatibility in all test concentrations for the imaging contrast. The biodistribution analysis revealed the highest serum concentration and circulation half-life for 12 nm-FePt, followed by 6 nm-FePt then 3 nm-FePt. Thus, the 3 nm-FePt showed higher brain concentrations. Anti-Her2 antibody conjugated FePt nanoparticles demonstrated molecular expression dependent CT/MRI dual imaging contrast effect in MBT2 cell line and its Her2/neu gene knock out counterpart. Selective contrast enhancement of Her2/neu overexpression cancer lesions in both CT and MRI was found in tumor bearing animal after tail vein injection of the nanoparticles. The 12 nm-FePt outperformed 3 nm-FePt in both imaging modalities. These results indicate the potential of FePt nanoparticles to serve as novel multimodal molecular imaging contrast agents in clinical settings.Keywords
This publication has 47 references indexed in Scilit:
- Polymer beacons for luminescence and magnetic resonance imaging of DNA deliveryProceedings of the National Academy of Sciences of the United States of America, 2009
- Controlled Growth and Magnetic Property of FePt Nanostructure: Cuboctahedron, Octapod, Truncated Cube, and CubeChemistry of Materials, 2009
- Interaction of Nanoparticles with CellsBiomacromolecules, 2009
- Nanoparticulate Assemblies of Amphiphiles and Diagnostically Active Materials for Multimodality ImagingAccounts of Chemical Research, 2009
- Alzheimer Disease: Quantitative Structural Neuroimaging for Detection and Prediction of Clinical and Structural Changes in Mild Cognitive ImpairmentRadiology, 2009
- A dual CT‐MR dendrimer contrast agent as a surrogate marker for convection‐enhanced delivery of intracerebral macromolecular therapeutic agentsContrast Media & Molecular Imaging, 2008
- Biomimetic amplification of nanoparticle homing to tumorsProceedings of the National Academy of Sciences of the United States of America, 2007
- Structural and functional MRI reveals multiple retinal layersProceedings of the National Academy of Sciences of the United States of America, 2006
- Calcium-sensitive MRI contrast agents based on superparamagnetic iron oxide nanoparticles and calmodulinProceedings of the National Academy of Sciences of the United States of America, 2006
- Nanomedicines and nanotoxicology: some physiological principlesOccupational Medicine, 2006