Excretion and folding of plasmalemma function to accommodate alterations in guard cell volume during stomatal closure in Vicia faba L.
Open Access
- 5 July 2010
- journal article
- research article
- Published by Oxford University Press (OUP) in Journal of Experimental Botany
- Vol. 61 (13), 3749-3758
- https://doi.org/10.1093/jxb/erq197
Abstract
Stomatal movement results in large and repetitive changes in cell volume and consequently surface area. While endocytosis has been extensively studied and is thought to be a major mechanism for accommodating the volume changes as evidenced mainly by fluorescent labelling and confocal imaging, studies at the ultrastructural level in intact guard cells of stomata regulated by natural factors have never been reported. Here, it is reported that excretion and folding of the plasmalemma are critical for accommodating the volume alterations in intact guard cells in Vicia faba L. Using transmission electron microscopy in combination with laser confocal microscopy, it was observed that in fully opened stomata the plasmalemma was smooth and tightly adhered to the cell walls while a whole large vacuole appeared in the cell. In the closed stomata, besides vacuole fragmentation, endocytosis of the tonoplast rather than the plasmalemma commonly occurred. Importantly, in stomata where pore closure was induced by circadian rhythm or CO2, numerous tiny vesicles were found outside the plasmalemma and, moreover, extensive folding of the plasmalemma could also be found in some regions of the cells. Additionally, an unknown structure was found at the interface between the plasmalemma and cell walls, especially in those areas of the cell where extensive folding occurred, suggesting that plasmalemma turnover is possibly associated with an interaction between the plasmalemma and cell walls. Collectively, the results strongly indicate that excretion and folding of the plasmalemma are critical for the accommodation of the cell volume alterations during stomatal movement.Keywords
This publication has 27 references indexed in Scilit:
- Membrane trafficking in guard cells during stomatal movementPlant Signaling & Behavior, 2008
- Guard Cells Elongate: Relationship of Volume and Surface Area during Stomatal MovementBiophysical Journal, 2007
- The Dynamic Changes of Tonoplasts in Guard Cells Are Important for Stomatal Movement inVicia fabaPlant Physiology, 2005
- Guard cells undergo constitutive and pressure-driven membrane turnoverProtoplasma, 2005
- Endocytosis against high turgor: intact guard cells of Vicia faba constitutively endocytose fluorescently labelled plasma membrane and GFP‐tagged K+‐channel KAT1The Plant Journal, 2004
- Changes in Surface Area of Intact Guard Cells Are Correlated with Membrane InternalizationPlant Physiology, 2003
- Mutations in Actin-Related Proteins 2 and 3 Affect Cell Shape Development in ArabidopsisPlant Cell, 2003
- Mechanical study of the deformation and rupture of the plasma membranes of protoplasts during osmotic expansionsThe Journal of Membrane Biology, 1986
- A low-viscosity epoxy resin embedding medium for electron microscopyJournal of Ultrastructure Research, 1969
- Plasmolysis and permeability of alpha-irradiated epidermal cells ofAllium cepa L.Protoplasma, 1966