Transcription of histone gene cluster by differential core-promoter factors
- 31 October 2007
- journal article
- Published by Cold Spring Harbor Laboratory in Genes & Development
- Vol. 21 (22), 2936-2949
- https://doi.org/10.1101/gad.1608807
Abstract
The 100 copies of tandemly arrayed Drosophila linker (H1) and core (H2A/B and H3/H4) histone gene cluster are coordinately regulated during the cell cycle. However, the molecular mechanisms that must allow differential transcription of linker versus core histones prevalent during development remain elusive. Here, we used fluorescence imaging, biochemistry, and genetics to show that TBP (TATA-box-binding protein)-related factor 2 (TRF2) selectively regulates the TATA-less Histone H1 gene promoter, while TBP/TFIID targets core histone transcription. Importantly, TRF2-depleted polytene chromosomes display severe chromosomal structural defects. This selective usage of TRF2 and TBP provides a novel mechanism to differentially direct transcription within the histone cluster. Moreover, genome-wide chromatin immunoprecipitation (ChIP)-on-chip analyses coupled with RNA interference (RNAi)-mediated functional studies revealed that TRF2 targets several classes of TATA-less promoters of >1000 genes including those driving transcription of essential chromatin organization and protein synthesis genes. Our studies establish that TRF2 promoter recognition complexes play a significantly more central role in governing metazoan transcription than previously appreciated.Keywords
This publication has 55 references indexed in Scilit:
- Supervised Detection of Conserved Motifs in DNA Sequences with CosmoStatistical Applications in Genetics and Molecular Biology, 2007
- Novel TRF1/BRF target genes revealed by genome-wide analysis of Drosophila Pol III transcriptionThe EMBO Journal, 2006
- Two Isoforms of Drosophila TRF2 Are Involved in Embryonic Development, Premeiotic Chromatin Condensation, and Proper Differentiation of Germ Cells of Both SexesMolecular and Cellular Biology, 2006
- Coactivator cross-talk specifies transcriptional outputGenes & Development, 2006
- DREF Is Required for Efficient Growth and Cell Cycle Progression in Drosophila Imaginal DiscsMolecular and Cellular Biology, 2005
- Diversified transcription initiation complexes expand promoter selectivity and tissue-specific gene expressionJournal of Bone and Joint Surgery, 2003
- TRF2 associates with DREF and directs promoter-selective gene expression in DrosophilaNature, 2002
- A Direct Approach to False Discovery RatesJournal of the Royal Statistical Society Series B: Statistical Methodology, 2002
- Spermiogenesis Deficiency in Mice Lacking the Trf2 GeneScience, 2001
- Distinct Roles for TBP and TBP-Like Factor in Early Embryonic Gene Transcription in XenopusScience, 2000