Transient Gene Expression in Serum-Free Suspension-Growing Mammalian Cells for the Production of Foot-and-Mouth Disease Virus Empty Capsids
Open Access
- 20 August 2013
- journal article
- research article
- Published by Public Library of Science (PLoS) in PLOS ONE
- Vol. 8 (8), e72800
- https://doi.org/10.1371/journal.pone.0072800
Abstract
Foot-and-mouth disease (FMD) is a highly contagious disease of cloven-hoofed animals. It produces severe economic losses in the livestock industry. Currently available vaccines are based on inactivated FMD virus (FMDV). The use of empty capsids as a subunit vaccine has been reported to be a promising candidate because it avoids the use of virus in the vaccine production and conserves the conformational epitopes of the virus. In this report, we explored transient gene expression (TGE) in serum-free suspension-growing mammalian cells for the production of FMDV recombinant empty capsids as a subunit vaccine. The recombinant proteins produced, assembled into empty capsids and induced protective immune response against viral challenge in mice. Furthermore, they were recognized by anti-FMDV bovine sera. By using this technology, we were able to achieve expression levels that are compatible with the development of a vaccine. Thus, TGE of mammalian cells is an easy to perform, scalable and cost-effective technology for the production of a recombinant subunit vaccine against FMDV.Keywords
This publication has 39 references indexed in Scilit:
- Rational Engineering of Recombinant Picornavirus Capsids to Produce Safe, Protective Vaccine AntigenPLoS Pathogens, 2013
- Development of a Foot-and-Mouth Disease Virus Serotype A Empty Capsid Subunit Vaccine Using Silkworm (Bombyx mori) PupaePLOS ONE, 2012
- A simplified polyethylenimine-mediated transfection process for large-scale and high-throughput applicationsMethods, 2011
- Research in advance for FMD Novel VaccinesVirology Journal, 2011
- Association between nasal shedding and fever that influenza A (H3N2) induces in dogsVirology Journal, 2011
- Foot-and-Mouth Disease Virus Assembly: Processing of Recombinant Capsid Precursor by Exogenous Protease Induces Self-Assembly of Pentamers In Vitro in a Myristoylation-Dependent MannerJournal of Virology, 2009
- Recombinant protein production by large-scale transient gene expression in mammalian cells: state of the art and future perspectivesBiotechnology Letters, 2007
- Development of novel strategies to control foot-and-mouth disease: Marker vaccines and antiviralsBiologicals, 2005
- FMD vaccinesVirus Research, 2003
- Stability and immunogenicity of empty particles of foot-and-mouth disease virusArchiv für die gesamte Virusforschung, 1979