Rapid Platelet Accumulation Leading to Thrombotic Occlusion
- 22 March 2011
- journal article
- Published by Springer Science and Business Media LLC in Annals of Biomedical Engineering
- Vol. 39 (7), 1961-1971
- https://doi.org/10.1007/s10439-011-0296-3
Abstract
Platelet thrombosis under arterial conditions remains a large clinical problem. Previous in vitro experiments have concentrated on early adherence without thrombotic occlusion. We have developed a controllable hemodynamic system that creates intravascular thrombosis to occlusion. Lightly heparinized (3.5 USP units/mL), whole, porcine blood is perfused through a 1.5 mm inner diameter, tubular, collagen-coated stenosis. The microscopic growth of thrombus is optically recorded using a high resolution CCD camera. Occlusive thrombus is examined using microcomputed tomography and histology. Thrombus consistently formed in the throat of the stenosis where wall shear rates were greatest. Rapid platelet accumulation (RPA) reached rates as high as 13.7 μm3 μm−2 min−1. Total occlusion of flow occurred after 17 ± 2.6 min (n = 6). The average thrombus volume accumulation of 7.8 ± 3.5 μm3 μm2 min−1 occurred under very high wall shear rates exceeding 100,000 s−1. Significant volumes of thrombus did not form until 7.6 ± 3.6 min after the onset of flow, a delay consistent with activation of adherent mural platelets. Platelets did not accumulate with large volume for normal wall shear rates −1. Very high wall shear rates stimulate the capture of millions of circulating platelets with exposure times <2 ms in an arterial stenosis.Keywords
This publication has 26 references indexed in Scilit:
- Wall shear over high degree stenoses pertinent to atherothrombosisJournal of Biomechanics, 2010
- The Role of the Platelet Glycoprotein IIb / IIIa in Thrombosis and HaemostasisCurrent Pharmaceutical Design, 2004
- Platelet Collagen ReceptorsThrombosis and Haemostasis, 2001
- Glycoprotein (GP) Ib-IX-transfected Cells Roll on a von Willebrand Factor Matrix under FlowPublished by Elsevier BV ,1999
- Fluid mechanics of arterial stenosis: Relationship to the development of mural thrombusAnnals of Biomedical Engineering, 1997
- Real-time analysis of shear-dependent thrombus formation and its blockade by inhibitors of von Willebrand factor binding to plateletsBlood, 1993
- Mathematical analysis of mural thrombogenesis. Concentration profiles of platelet-activating agents and effects of viscous shear flowBiophysical Journal, 1989
- Mechanisms of arterial thrombosis in nonparallel streamlines: platelet thrombi grow on the apex of stenotic severely injured vessel wall. Experimental study in the pig model.JCI Insight, 1989
- Influence of arterial damage and wall shear rate on platelet deposition. Ex vivo study in a swine model.Arteriosclerosis: An Official Journal of the American Heart Association, Inc., 1986
- Thrombosis and Acute Coronary-Artery Lesions in Sudden Cardiac Ischemic DeathNew England Journal of Medicine, 1984