Comparative Proteome Analysis of Shewanella putrefaciens WS13 Mature Biofilm Under Cold Stress
Open Access
- 9 June 2020
- journal article
- research article
- Published by Frontiers Media SA in Frontiers in Microbiology
- Vol. 11, 1225
- https://doi.org/10.3389/fmicb.2020.01225
Abstract
Worldwide, Shewanella putrefaciens is the predominant seafood spoilage microorganism during cold storage. This bacterium can attach to biotic/abiotic surfaces to form biofilms which contribute to seafood quality degradation and shelf-life reduction. The mechanism of S. putrefaciens biofilm formation is not yet described. Crystal violet staining in combination with confocal laser scanning microscopy (CLSM) was used to study the sequence of events leading to the establishment of a mature biofilm at 4, 15, and 30°C. In addition, the main chemical constituents of the mature biofilm were determined by Raman spectroscopy (RM), whereas, comparative proteomic analysis was used to quantify changes in metabolic pathways and to find out underlying protein determinants. The physical dimensions of the mature biofilm, i.e., biomass, biovolume, and mean thickness, were higher at 4°C when compared to 15 and 30°C. The variations of proteins measured by RM confirmed the importance of proteins during the formation of a mature biofilm. Comparative proteomic analysis showed that siderophore and iron chelate transport proteins were down-regulated during mature biofilm formation. The down-regulated aforementioned proteins are involved in promoting iron storage in response to a higher demand for metabolic energy, whereas, the upregulated proteins of the sulfur relay system, pyrimidine metabolism, and purine metabolism are related to bacterial adaptability. Synthesis of proteins related to cold stress was increased and proteins involved in aminoacyl-tRNA biosynthesis were up-regulated, whereas, proteins involved in aminopeptidase activity were down-regulated. Proteolysis to scavenge energy was reduced as proteins involved in pyrophosphatase activity were up-regulated. Also extracellular eDNA was found which may play an important role in maintaining the stability of mature S. putrefaciens biofilm structures under cold stress. This work provides a better understanding of the role of proteins in mature biofilms. In addition, the biofilm formation mechanism of a psychrotrophic spoilage bacterial species at low temperature is explored, which may contribute to generating biofilm controlling strategies during seafood preservation and processing.Funding Information
- National Natural Science Foundation of China
- Science and Technology Commission of Shanghai Municipality
This publication has 44 references indexed in Scilit:
- Proteomics Reveals a Role for the RNA Helicase crhR in the Modulation of Multiple Metabolic Pathways during Cold Acclimation of Synechocystis sp. PCC6803Journal of Proteome Research, 2011
- Biofilm formation and dispersal in Gram-positive bacteriaCurrent Opinion in Biotechnology, 2011
- Extracellular DNA is abundant and important for microcolony strength in mixed microbial biofilmsEnvironmental Microbiology, 2010
- Extraction and Analysis of Extracellular Polymeric Substances: Comparison of Methods and Extracellular Polymeric Substance Levels inSalmonella pullorumSA 1685Environmental Engineering Science, 2009
- Assembly and Development of the Pseudomonas aeruginosa Biofilm MatrixPLoS Pathogens, 2009
- In Situ Surface-Enhanced Raman Scattering Analysis of BiofilmAnalytical Chemistry, 2008
- Bacillus Spore Classification via Surface-Enhanced Raman Spectroscopy and Principal Component AnalysisApplied Spectroscopy, 2008
- Comparison of Psychro-Active Arctic Marine Bacteria and Common Mesophillic Bacteria Using Surface-Enhanced Raman SpectroscopyApplied Spectroscopy, 2005
- Suppression of bacterial biofilm formation by iron limitationMedical Hypotheses, 2004
- Interaction between fish spoilage bacteria Pseudomonas sp. and Shewanella putrefaciens in fish extracts and on fish tissueJournal of Applied Bacteriology, 1996