A new family of lipolytic plant enzymes with members in rice, arabidopsis and maize
- 27 December 1995
- journal article
- research article
- Published by Wiley in FEBS Letters
- Vol. 377 (3), 475-480
- https://doi.org/10.1016/0014-5793(95)01405-5
Abstract
We have noted a striking similarity between the sequences of proteins in a novel family of lipases we recently reported [Upton, C. and Buckley, J.T. (1995) Trends Biol. Sci. 20, 178–9] and more than 120 sequences from the database of Expressed Sequence Tags (dbEST) which correspond to at least 30 unique genes from arabidopsis, rice and maize. A cDNA (Arab-1) corresponding to one of these sequences was isolated, sequenced and translated. There was significant similarity to sequences in the new lipase family over the entire open reading frame of Arab-1 and when expressed in E. coli, the gene product was lipolytic. Arab-1 and genes for some of the other plant proteins appear to be differentially expressed. They may play a role in the regulation of lipid metabolism during plant developmentKeywords
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