Mapping of steroid 21-hydroxylase genes adjacent to complement component C4 genes in HLA, the major histocompatibility complex in man.
- 1 January 1985
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 82 (2), 521-525
- https://doi.org/10.1073/pnas.82.2.521
Abstract
The genes for 4 components (C) of complement in the HLA were aligned previously in a series of overlapping cosmid cloned inserts. Those inserts, which contained the 2 C4 genes and C4A and C4B, hybridized with human adrenal mRNA, indicating that they contain a gene expressed in the adrenal. The mRNA fraction of 2.4 kilobases (kb) hybridizes with genomic DNA of 4.5 kb, which is duplicated and lies about 1.5 kb 3'' of both the C4A and the C4B complement genes. Sequencing of a 430-base section and comparison with the published [complement] cDNA sequence of bovine cytochrome P-450 21-hydroxylase, peptide sequences of porcine 21-hydroxylase, and a cDNA sequence of a rat liver cytochrome P-450 identified the gene as coding for human steroid 21-hydroxylase [steroid,hydrogen-donor:oxygen oxidoreductase (21-hydroxylating), EC 1.14.99.10]. Mapping of the gene was helped by use of a synthetic oligonucleotide based on the bovine cDNA sequence.This publication has 21 references indexed in Scilit:
- Structure and organization of theC4genesPhilosophical Transactions of the Royal Society of London. B, Biological Sciences, 1984
- The structural basis of the multiple forms of human complement component C4Cell, 1984
- A molecular map of the human major histocompatibility complex class III region linking complement genes C4, C2 and factor BNature, 1984
- Report of the committee on the genetic constitution of chromosome 6Cytogenetic and Genome Research, 1984
- The construction of cosmid libraries which can be used to transform eukaryotic cellsNucleic Acids Research, 1982
- Polymorphic DNA region adjacent to the 5' end of the human insulin gene.Proceedings of the National Academy of Sciences, 1981
- [57] Sequencing end-labeled DNA with base-specific chemical cleavagesMethods in enzymology, 1980
- Isolation of biologically active ribonucleic acid from sources enriched in ribonucleaseBiochemistry, 1979
- Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase IJournal of Molecular Biology, 1977
- Detection of specific sequences among DNA fragments separated by gel electrophoresisJournal of Molecular Biology, 1975