Small molecule-induced ablation and subsequent regeneration of larval zebrafish melanocytes
- 15 September 2006
- journal article
- Published by The Company of Biologists in Development
- Vol. 133 (18), 3563-3573
- https://doi.org/10.1242/dev.02533
Abstract
We developed a method to efficiently ablate a single cell type, the zebrafish melanocyte, and study the mechanisms of its regeneration. We found that a small molecule, (2-morpholinobutyl)-4-thiophenol (MoTP), specifically ablates zebrafish larval melanocytes or melanoblasts, and that this melanocytotoxicity is dependent on tyrosinase activity, which presumably converts MoTP to cytotoxic quinone species. Following melanocyte ablation by MoTP treatment, we demonstrate by BrdU incorporation experiments that regenerated melanocytes are derived from the division of otherwise quiescent melanocyte precursors or stem cells. We further show that larval melanocyte regeneration requires the kit receptor tyrosine kinase. Our results suggest that a small number of melanocyte precursors or stem cells unevenly distributed in larvae are drawn upon to reconstitute the larval melanocyte population following melanocyte ablation by MoTP.Keywords
This publication has 50 references indexed in Scilit:
- Neomycin-Induced Hair Cell Death and Rapid Regeneration in the Lateral Line of Zebrafish ( Danio rerio )Journal of the Association for Research in Otolaryngology, 2003
- Dominant role of the niche in melanocyte stem-cell fate determinationNature, 2002
- Cell proliferation and movement during early fin regeneration in zebrafishDevelopmental Dynamics, 2001
- The Biology of Hematopoietic Stem CellsAnnual Review of Cell and Developmental Biology, 1995
- Stages of embryonic development of the zebrafishDevelopmental Dynamics, 1995
- Phase I study of intravenous 4-hydroxyanisoleEuropean Journal Of Cancer, 1992
- Initial Mushroom Tyrosinase-Catalysed Oxidation Product of 4-Hydroxyanisole is 4-Methoxy-Ortho-BenzoquinonePigment Cell Research, 1988
- Radicals and melanomasPhilosophical Transactions of the Royal Society of London. B, Biological Sciences, 1985
- Abnormal Cell Proliferation in Psoriasis**From the Department of Dermatology, University of Miami School of Medicine, Miami, Florida.Journal of Investigative Dermatology, 1968
- Origin of the blastema in regenerating limbs of the newt Triturus viridescensDevelopmental Biology, 1961