High frequency of centromere inactivation resulting in stable dicentric chromosomes of maize
Open Access
- 21 February 2006
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences of the United States of America
- Vol. 103 (9), 3238-3243
- https://doi.org/10.1073/pnas.0509650103
Abstract
Somatic chromosome spreads from maize (Zea mays L.) plants containing B-A translocation chromosomes undergoing the chromosome type breakage–fusion–bridge cycle were examined by FISH. The size and type of extra chromosomes varied among cells of the same individual. A collection of minichromosomes derived from the chromosome type breakage–fusion–bridge cycle was examined for the presence of stable dicentric chromosomes. Six of 23 chromosomes in the collection contained two regions with DNA sequences typical of centromeres. Functional analysis and immunolabeling of CENH3, the centromere-specific histone H3 variant, revealed only one functional centromere per chromosome, despite the duplicate centromere sequences. One plant was found with an inactive B centromere that had been translocated to the short arm of chromosome 9. The translocated centromere region appeared identical to that of a normal B chromosome. The inactivation of the centromeres was stable for at least four generations. By using dicentrics from dispensable chromosomes, centromere inactivation was found to be quite common under these circumstances.Keywords
This publication has 12 references indexed in Scilit:
- Stable barley chromosomes without centromeric repeatsProceedings of the National Academy of Sciences of the United States of America, 2005
- Molecular and Functional Dissection of the Maize B Chromosome CentromerePlant Cell, 2005
- Minichromosomes derived from the B chromosome of maizeCytogenetic and Genome Research, 2005
- Sequences associated with A chromosome centromeres are present throughout the maize B chromosomeChromosoma, 2004
- Chromosome painting using repetitive DNA sequences as probes for somatic chromosome identification in maizeProceedings of the National Academy of Sciences of the United States of America, 2004
- B chromosomes in plants: escapees from the A chromosome genome?Trends in Plant Science, 2003
- Immunogold labeling of chromosomes for scanning electron microscopy: a closer look at phosphorylated histone H3 in mitotic metaphase chromosomes of Hordeum vulgare.Chromosome Research, 2003
- Determining centromere identity: cyclical stories and forking pathsNature Reviews Genetics, 2001
- Phosphorylation of histone H3 is correlated with changes in the maintenance of sister chromatid cohesion during meiosis in maize, rather than the condensation of the chromatinJournal of Cell Science, 2000
- The Behavior in Successive Nuclear Divisions of a Chromosome Broken at MeiosisProceedings of the National Academy of Sciences of the United States of America, 1939