Differential inflammatory mediator response in vitro from murine macrophages to lactobacilli and pathogenic intestinal bacteria
- 4 April 2007
- journal article
- research article
- Published by Wiley in International Journal of Experimental Pathology
- Vol. 88 (3), 155-164
- https://doi.org/10.1111/j.1365-2613.2007.00530.x
Abstract
Chronic active colitis (including inflammatory bowel disease - IBD) is maintained by a variety of pro-inflammatory mediators. Certain intestinal bacterial strains may induce colitis, whereas some strains (e.g. Lactobacillus spp.) show a protective effect in colitis owing to their anti-inflammatory activity. In this study, we have examined the production of selected inflammatory cytokines, reactive oxygen species (ROS), nitric oxide (NO) and the expression of haeme oxygenase-1 (HO-1) by murine peritoneal macrophages stimulated in vitro by the intestinal bacterial strains, isolated from mice with colitis. Lactobacillus strains (Lactobacillus reuteri, L. johnsonii, L. animalis/murinus) and two potentially pathogenic bacteria (Escherichia coli and Enterococcus faecalis) induced the production of substantial amounts of cytokines with a strain specific profile. Despite some interstrain differences, all lactobacilli induced production of anti-inflammatory cytokines (IL-10(high), IL-6(low), IL-12p70(low)). Conversely, E. faecalis and E. coli induced the production of proinflammatory cytokines (TNF-alpha, IL-12p70), the cytokines essential for chronic IBD. Macrophages released comparably substantial amounts of ROS in response to all Lactobacillus strains tested, while E. coli and E. faecalis ability to induce generation of ROS was negligible. In contrast to ROS, the production of NO/NO(2) (-) by macrophages activated with all bacterial strains tested was similar. Moreover, for the first time, it has been shown that intestinal bacteria differed in their ability to induce expression of HO-1, a stress-inducible enzyme with antioxidant and anti-inflammatory properties. The beneficial immunoregulatory properties of candidate probiotic bacteria for the treatment of IBD are discussed.This publication has 47 references indexed in Scilit:
- Strain-Specific Effects of Probiotics on Gut Barrier Integrity following Hemorrhagic ShockInfection and Immunity, 2005
- Taurine chloramine and taurine bromamine induce heme oxygenase-1 in resting and LPS-stimulated J774.2 macrophagesAmino Acids, 2004
- An interleukin 12 p40-IgG2b fusion protein abrogates T cell mediated inflammation: anti-inflammatory activity in Crohn's disease and experimental colitis in vivoGut, 2004
- The role of endogenous bacterial floraEuropean Journal of Gastroenterology & Hepatology, 2003
- Intestinal oxidative damage in inflammatory bowel disease: semi‐quantification, localization, and association with mucosal antioxidantsThe Journal of Pathology, 2003
- Lipoteichoic Acids fromLactobacillusStrains Elicit Strong Tumor Necrosis Factor Alpha-Inducing Activities in Macrophages through Toll-Like Receptor 2Clinical and Vaccine Immunology, 2003
- Intestinal barrier functionCurrent Opinion in Clinical Nutrition and Metabolic Care, 2002
- Heme oxygenase-1 mediates the anti-inflammatory effect of interleukin-10 in miceNature Medicine, 2002
- Blockade of NF- κ B Activation and Donation of Nitric Oxide: New Treatment Options in Inflammatory Bowel Disease?Scandinavian Journal of Gastroenterology, 2002
- Ulcerative colitis and adenocarcinoma of the colon in Gαi2-deficient miceNature Genetics, 1995