In vitro and ex vivo strategies for intracellular delivery
Top Cited Papers
- 12 October 2016
- journal article
- review article
- Published by Springer Science and Business Media LLC in Nature
- Vol. 538 (7624), 183-192
- https://doi.org/10.1038/nature19764
Abstract
Intracellular delivery of materials has become a critical component of genome-editing approaches, ex vivo cell-based therapies, and a diversity of fundamental research applications. Limitations of current technologies motivate development of next-generation systems that can deliver a broad variety of cargo to diverse cell types. Here we review in vitro and ex vivo intracellular delivery approaches with a focus on mechanisms, challenges and opportunities. In particular, we emphasize membrane-disruption-based delivery methods and the transformative role of nanotechnology, microfluidics and laboratory-on-chip technology in advancing the field.Keywords
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