A Point Mutation of Adh1 Gene is Involved in the Repression of Coleoptile Elongation under Submergence in Rice
Open Access
- 1 January 2006
- journal article
- Published by Japanese Society of Breeding in Breeding Science
- Vol. 56 (1), 69-74
- https://doi.org/10.1270/jsbbs.56.69
Abstract
In higher plants, alcoholic fermentation is required to supply NAD+ to the glycolytic pathway, which is responsible for ATP synthesis under anaerobic conditions. Matsumura et al. (1995) previously isolated the reduced adh activity (rad) mutant in rice, in which elongation of the coleoptile is repressed under submergence. However, the rad gene had not been characterized. In the present study, we observed that, in the coleoptile, the Adh1 mRNA levels were comparable between the rad mutant and the wild type cultivar Kinmaze, while the amount of ADH1 protein was much lower in the rad mutant than in the wild type. Sequencing showed that G106 of the Adh1 gene of Kinmaze was replaced with A in the rad mutant, resulting in an E36K substitution in the deduced amino acid sequence. A genotyping experiment using F2 plants from a cross of the rad mutant with the indica cultivar Kasalath indicated that the point mutation was involved in the repression of coleoptile elongation. Furthermore, since the reduced ADH activity appeared to cause an ATP deficiency, elongation of the coleoptile was repressed in the submerged rad mutant.Keywords
This publication has 24 references indexed in Scilit:
- Introduction of the Long-Coleoptile Trait to Improve the Establishment of Direct-Seeded Rice in Submerged Fields in Cool Climates.Plant Production Science, 2002
- Submergence Tolerance and Ethanolic Fermentation In Rice ColeoptilesPlant Production Science, 2001
- A Varietal Difference in Coleoptile Growth is Correlated with Seedling Establishment of Direct Seeded Rice in Submerged Field under Low-Temperature Conditions.Plant Production Science, 2001
- Expression of a Gene Encoding Mitochondrial Aldehyde Dehydrogenase in Rice Increases under Submerged ConditionsPlant Physiology, 2000
- SWISS‐MODEL and the Swiss‐Pdb Viewer: An environment for comparative protein modelingElectrophoresis, 1997
- A Rice Mutant Lacking Alcohol Dehydrogenase.Ikushugaku zasshi, 1995
- MAIZE AdhlAnnual Review of Genetics, 1985
- THE EFFECT OF A MUTATION CAUSING ALCOHOL DEHYDROGENASE DEFICIENCY ON FLOODING TOLERANCE IN BARLEYNew Phytologist, 1982
- Soluble Sugars, Respiration, and Energy Charge during Aging of Excised Maize Root TipsPlant Physiology, 1980
- An Example of Gene Fixation Resulting from Selective Advantage in Suboptimal ConditionsThe American Naturalist, 1969