Panduratin A Inhibits the Growth of A549 Cells through Induction of Apoptosis and Inhibition of NF-KappaB Translocation
Open Access
- 20 March 2011
- Vol. 16 (3), 2583-2598
- https://doi.org/10.3390/molecules16032583
Abstract
In the present study we investigated the effects of panduratin A, isolated from Boesenbergia rotunda, on proliferation and apoptosis in A549 human non-small cell lung cancer cells. Cell proliferation and induction of apoptosis was determined by the real-time cellular analyzer (RTCA), MTT assay and High Content Screening (HCS). The RTCA assay indicated that panduratin A exhibited cytotoxicity, with an IC50 value of 4.4 µg/mL (10.8 µM). Panduratin A arrested cancer cells labeled with bromodeoxyuridine (BrdU) and phospho-Histone H3 in the mitotic phase. The cytotoxic effects of panduratin A were found to be accompanied by a dose-dependent induction of apoptosis, as assessed by DNA condensation, nuclear morphology and intensity, cell permeability, mitochondrial mass/ potential, F-actin and cytochrome c. In addition, treatment with an apoptosis-inducing concentration of panduratin A resulted in significant inhibition of Nuclear Factor-kappa Beta (NF-κB) translocation from cytoplasm to nuclei activated by tumor necrosis factor-alpha (TNF-α), as illustrated by the HCS assay. Our study provides evidence for cell growth inhibition and induction of apoptosis by panduratin A in the A549 cell line, suggesting its therapeutic potential as an NF-κB inhibitor.Keywords
This publication has 26 references indexed in Scilit:
- Potential pharmacological control of the NF-κB pathwayTrends in Pharmacological Sciences, 2009
- Induction of apoptosis and cell cycle arrest by a chalcone panduratin A isolated from Kaempferia pandurata in androgen-independent human prostate cancer cells PC3 and DU145Carcinogenesis: Integrative Cancer Research, 2006
- A role for actin in aging and apoptosisBiochemical Society Transactions, 2005
- Induction of Apoptosis by Panduratin A Isolated fromKaempferia panduratain Human Colon Cancer HT-29 CellsPlanta Medica, 2005
- Licochalcone-A, a novel flavonoid isolated from licorice root (Glycyrrhiza glabra), causes G2 and late-G1 arrests in androgen-independent PC-3 prostate cancer cellsBiochemical and Biophysical Research Communications, 2004
- Nuclear factor-κBCancer Cell, 2004
- ATR and ATRIP: Partners in Checkpoint SignalingScience, 2001
- Mitochondria and apoptosisEuropean Journal of Biochemistry, 1998
- High-Content Screening: A New Approach to Easing Key Bottlenecks in the Drug Discovery ProcessSLAS Discovery, 1997
- (1'RS,2'SR,6'RS)-(2,6-Dihydroxy-4-methoxyphenyl)-[3'-methyl-2'-(3''-methylbut-2''-enyl)-6'-phenyl-cyclohex-3'-enyl]methanone (panduratin A)-a Constituent of the Red Rhizomers of a variety of Boesenbergia pandurataAustralian Journal of Chemistry, 1984