Human antibodies from immunized donors are protective against anthrax toxin in vivo
- 12 October 2003
- journal article
- Published by Springer Science and Business Media LLC in Nature Biotechnology
- Vol. 21 (11), 1305-1306
- https://doi.org/10.1038/nbt891
Abstract
A panel of Fabs that neutralize anthrax toxin in vitro was selected from libraries generated from human donors vaccinated against anthrax. At least two of these antibodies protect rats from anthrax intoxication in vivo. Fabs 83K7C and 63L1D bind with subnanomolar affinity to protective antigen (PA) 63, and Fab 63L1D neutralizes toxin substoichiometrically, inhibits lethal factor (LF) interaction with PA63 and binds to a conformational epitope formed by PA63.Keywords
This publication has 10 references indexed in Scilit:
- Stoichiometry of Anthrax Toxin ComplexesBiochemistry, 2001
- CLAMP©: a biosensor kinetic data analysis programTrends in Biochemical Sciences, 1998
- Passive protection by polyclonal antibodies against Bacillus anthracis infection in guinea pigsInfection and Immunity, 1997
- Anthrax protective antigen forms oligomers during intoxication of mammalian cells.Journal of Biological Chemistry, 1994
- Anthrax toxin protective antigen is activated by a cell surface protease with the sequence specificity and catalytic properties of furin.Proceedings of the National Academy of Sciences, 1992
- Production and characterization of monoclonal antibodies against the lethal factor component of Bacillus anthracis lethal toxinInfection and Immunity, 1990
- Production and characterization of monoclonal antibodies to the protective antigen component of Bacillus anthracis toxinInfection and Immunity, 1988