Negative Regulation of Fibroblast Motility by Ena/VASP Proteins
- 1 June 2000
- journal article
- research article
- Published by Elsevier BV in Cell
- Vol. 101 (7), 717-728
- https://doi.org/10.1016/s0092-8674(00)80884-3
Abstract
Ena/VASP proteins have been implicated in cell motility through regulation of the actin cytoskeleton and are found at focal adhesions and the leading edge. Using overexpression, loss-of-function, and inhibitory approaches, we find that Ena/VASP proteins negatively regulate fibroblast motility. A dose-dependent decrease in movement is observed when Ena/VASP proteins are overexpressed in fibroblasts. Neutralization or deletion of all Ena/VASP proteins results in increased cell movement. Selective depletion of Ena/VASP proteins from focal adhesions, but not the leading edge, has no effect on motility. Constitutive membrane targeting of Ena/VASP proteins inhibits motility. These results are in marked contrast to current models for Ena/VASP function derived mainly from their role in the actin-driven movement of Listeria monocytogenes.This publication has 37 references indexed in Scilit:
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