Teriparatide (1‐34 human PTH) regulation of osterix during fracture repair
- 21 August 2008
- journal article
- research article
- Published by Wiley in Journal of Cellular Biochemistry
- Vol. 105 (1), 219-226
- https://doi.org/10.1002/jcb.21816
Abstract
Based on remarkable success of PTH as an anabolic drug for osteoporosis, case reports of off‐label use of teriparatide (1‐34 PTH) in patients with complicated fractures and non‐unions are emerging. We investigated the mechanisms underlying PTH accelerated fracture repair. Bone marrow cells from 7 days 40 µg/kg of teriparatide treated or saline control mice were cultured and Osx and osteoblast phenotypic gene expression assessed by real‐time RT‐PCR in these cells. Fractured animals injected daily with either saline or 40 µg/kg of teriparatide for up to 21 days were X‐rayed and histological assessment performed, as well as immunohistochemical analyses of the Osx expression in the fracture callus. Osx, Runx2 and osteoblast or chondrocyte phenotypic gene expression was also assessed in fracture calluses. Our data shows that Osx and Runx2 are up‐regulated in marrow‐derived MSCs isolated from mice systemically treated with teriparatide. Furthermore, these MSCs undergo accelerated osteoblast maturation compared to saline injected controls. Systemic teriparatide treatments also accelerated fracture healing in these mice concomitantly with increased Osx expression in the PTH treated fracture calluses compared to controls. Collectively, these data suggest a mechanism for teriparatide mediated fracture healing possibly via Osx induction in MSCs. J. Cell. Biochem. 105: 219–226, 2008.This publication has 40 references indexed in Scilit:
- Runx3/AML2/Cbfa3 Regulates Early and Late Chondrocyte DifferentiationJournal of Bone and Mineral Research, 2007
- Osterix/Sp7 regulates mesenchymal stem cell mediated endochondral ossificationJournal of Cellular Physiology, 2007
- Changes in Osteoblast, Chondrocyte, and Adipocyte Lineages Mediate the Bone Anabolic Actions of PTH and Small Molecule GSK‐3 InhibitorJournal of Cellular Biochemistry, 2007
- Bone RemodelingAnnals of the New York Academy of Sciences, 2006
- Osterix enhances proliferation and osteogenic potential of bone marrow stromal cellsBiochemical and Biophysical Research Communications, 2006
- Multiple Mechanisms Are Involved in Inhibition of Osteoblast Differentiation by PTHrP and PTH in KS483 CellsJournal of Bone and Mineral Research, 2005
- Effects of low-dose, intermittent treatment with recombinant human parathyroid hormone (1–34) on chondrogenesis in a model of experimental fracture healingBone, 2005
- Fracture healing as a post‐natal developmental process: Molecular, spatial, and temporal aspects of its regulationJournal of Cellular Biochemistry, 2003
- Effects of Daily Treatment with Parathyroid Hormone on Bone Microarchitecture and Turnover in Patients with Osteoporosis: A Paired Biopsy StudyJournal of Bone and Mineral Research, 2001
- The worldwide problem of osteoporosis: Insights afforded by epidemiologyBone, 1995