Novel Polymorphic Region of the rpoB Gene Containing Mycobacterium Species-Specific Sequences and Its Use in Identification of Mycobacteria
- 1 May 2003
- journal article
- research article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 41 (5), 2213-2218
- https://doi.org/10.1128/jcm.41.5.2213-2218.2003
Abstract
Sequence analysis of a specific region of the mycobacterium rpoB gene in 35 mycobacterial strains representing 26 different mycobacterial species of clinical importance showed that there exists a highly polymorphic region. Based on the sequences of the polymorphic region, the oligonucleotide probes of 14 mycobacterial species with relatively high clinical importance were designed and shown to be specific to their corresponding mycobacterial species by dot blot hybridization. The results showed that the probes designed in this study are highly specific to each mycobacterial species, which suggests that these sequences may be useful for the species identification of mycobacteria.Keywords
This publication has 10 references indexed in Scilit:
- Differentiation of Mycobacterial Species by PCR-Restriction Analysis of DNA (342 Base Pairs) of the RNA Polymerase Gene ( rpoB )Journal of Clinical Microbiology, 2001
- Simultaneous Genotyping and Species Identification Using Hybridization Pattern Recognition Analysis of Generic Mycobacterium DNA ArraysGenome Research, 1998
- Routine rapid Mycobacterium species assignment based on species-specific allelic variation in the 65-kilodalton heat shock protein gene (hsp65).1997
- NONTUBERCULOUS MYCOBACTERIAL INFECTIONSMedical Clinics of North America, 1997
- Mycobacterium interjectum, a new species isolated from a patient with chronic lymphadenitisJournal of Clinical Microbiology, 1993
- Mycobacterium aviumComplex Infection in the Acquired Immunodeficiency SyndromeThe New England Journal of Medicine, 1991
- Differentiation of Mycobacterium species by direct sequencing of amplified DNAJournal of General Microbiology, 1990
- Multiple sequence alignment with hierarchical clusteringNucleic Acids Research, 1988
- Use of Serology and Thin-Layer Chromatography for the Assembly of an Authenticated Collection of Serovars Within the Mycobacterium avium-Mycobacterium intracellulare-Mycobacterium scrofulaceum ComplexInternational Journal of Systematic and Evolutionary Microbiology, 1983
- Thin-layer chromatography of mycobacterial lipids as an aid to classification: Technical procedures; Mycobacterium fortuitumTubercle, 1965