Acute 40 percent exchange‐transfusion with hemoglobin‐vesicles (HbV) suspended in recombinant human serum albumin solution: degradation of HbV and erythropoiesis in a rat spleen for 2 weeks
- 1 March 2006
- journal article
- Published by Wiley in Transfusion
- Vol. 46 (3), 339-347
- https://doi.org/10.1111/j.1537-2995.2006.00727.x
Abstract
BACKGROUND: Hemoglobin-vesicles (HbVs; diameter, 251 ± 81 nm) are artificial O2 carriers. Their efficacy for acute exchange transfusion has been characterized in animal models. However subsequent profiles of recovery involving the degradation of HbV in the reticuloendothelial system (RES) and hematopoiesis remain unknown. STUDY DESIGN AND METHODS: Isovolemic 40 percent exchange transfusion was performed in 60 male Wistar rats with HbV suspended in 5 g per dL recombinant human serum albumin (rHSA; HbV/rHSA, [Hb] = 8.6 g/dL), stored rat RBCs suspended in rHSA (sRBC/rHSA), or rHSA alone. Hematological and plasma biochemical analyses and histopathological examination focusing on the spleen were conducted for the subsequent 14 days. RESULTS: The reduced hematocrit (Hct) level (26%) for the HbV/rHSA and rHSA groups returned to its original level (43%) in 7 days. Plasma erythropoietin was elevated in all groups: the rHSA group showed the highest value on Day 1 (321 ± 123 mIU/mL) relating to the anemic conditions (HbV/rHSA, 153 ± 22; sRBC/rHSA, 63 ± 7; baseline, 21 ± 3). Simultaneously, splenomegaly occurred in all the groups as HbV/rHSA > rHSA > sRBC/rHSA. Histopathologically, the accumulated HbV in the spleen was undetectable by Day 14, but hemosiderin was deposited in slight quantities for both the HbV/rHSA and sRBC/rHSA groups. Considerable amounts of erythroblasts were apparent in the spleens of both the rHSA and the HbV/rHSA groups. CONCLUSION: HbVs were phagocytized and degraded in RES, a physiological compartment for the degradation of RBCs, and the elevated erythropoietic activity resulted in the complete recovery of Hct within 7 days in the rat model.Keywords
This publication has 39 references indexed in Scilit:
- Hemorrhagic Shock Resuscitation With an Artificial Oxygen Carrier, Hemoglobin Vesicle, Maintains Intestinal Perfusion and Suppresses the Increase in Plasma Tumor Necrosis Factor-αASAIO Journal, 2004
- Physiological Capacity of the Reticuloendothelial System for the Degradation of Hemoglobin Vesicles (Artificial Oxygen Carriers) after Massive Intravenous Doses by Daily Repeated Infusions for 14 DaysJournal of Pharmacology and Experimental Therapeutics, 2004
- Hemoglobin-vesicles suspended in recombinant human serum albumin for resuscitation from hemorrhagic shock in anesthetized rats*Critical Care Medicine, 2004
- Detection of lipopolysaccharide in hemoglobin‐vesicles by Limulus amebocyte lysate test with kinetic–turbidimetric gel clotting analysis and pretreatment of surfactantJournal of Pharmaceutical Sciences, 2004
- Metabolism of hemoglobin-vesicles (artificial oxygen carriers) and their influence on organ functions in a rat modelBiomaterials, 2003
- Redox cycling of diaspirin cross-linked hemoglobin induces G2/M arrest and apoptosis in cultured endothelial cellsBlood, 2001
- Hemoglobin-Vesicles as Oxygen CarriersThe American Journal of Pathology, 2001
- Oxygenation in tumors by modified hemoglobinsJournal of Surgical Oncology, 1996
- Characterization of two heme oxygenase isoforms in rat spleen: Comparison with the hematin-induced and constitutive isoforms of the liverBiochemical and Biophysical Research Communications, 1986
- Perspectives in Iron MetabolismNew England Journal of Medicine, 1982