Flow cytometric analysis of the recruitment of G0 cells in human epidermis in vivo following tape stripping
- 31 December 1986
- journal article
- research article
- Published by Wiley in Cell Proliferation
- Vol. 20 (1), 99-107
- https://doi.org/10.1111/j.1365-2184.1987.tb01086.x
Abstract
Tape stipping of human skin elicits a proliferative response of a synchronously-dividing group of cells. The progress of this cohort of cells has been monitored using two windows in the cell cycle, one located in mid-S phase and the other centred around G2 + M. The cellular DNA is measured with flow cytometry, the windows are defined by two ranges in the DNA histogram. The cohort can be described as the recuitment of cells from a pre-existing G0 compartment which consists of 76% of all proliferative cells. The duration of the S phase is calculated to be 10.2 hr and G2 + M phase 5.1 hr. The cell cycle time of 39 hr for normal human keratinocytes derived from these figures is in line with recent values obtained by different techniques.This publication has 11 references indexed in Scilit:
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