LasI/R and RhlI/R Quorum Sensing in a Strain ofPseudomonas aeruginosaBeneficial to Plants
- 1 August 2009
- journal article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 75 (15), 5131-5140
- https://doi.org/10.1128/aem.02914-08
Abstract
Pseudomonas aeruginosa possesses three quorum-sensing (QS) systems which are key in the expression of a large number of genes, including many virulence factors. Most studies of QS in P. aeruginosa have been performed in clinical isolates and have therefore focused on its role in pathogenicity. P. aeruginosa, however, is regarded as a ubiquitous organism capable of colonizing many different environments and also of establishing beneficial associations with plants. In this study we examined the role of the two N-acyl homoserine lactone systems known as RhlI/R and LasI/R in the environmental rice rhizosphere isolate P. aeruginosa PUPa3. Both the Rhl and Las systems are involved in the regulation of plant growth-promoting traits. The environmental P. aeruginosa PUPa3 is pathogenic in two nonmammalian infection models, and only the double las rhl mutants are attenuated for virulence. In fact it was established that the two QS systems are not hierarchically organized and that they are both important for the colonization of the rice rhizosphere. This is an in-depth genetic and molecular study of QS in an environmental P. aeruginosa strain and highlights several differences with QS regulation in the clinical isolate PAO1.Keywords
This publication has 65 references indexed in Scilit:
- Development of Galleria mellonella as an Alternative Infection Model for the Burkholderia cepacia ComplexInfection and Immunity, 2008
- Involvement of a Quorum-Sensing-Regulated Lipase Secreted by a Clinical Isolate of Burkholderia glumae in Severe Disease Symptoms in RiceApplied and Environmental Microbiology, 2007
- Environmental Regulation of Pseudomonas aeruginosa PAO1 Las and Rhl Quorum-Sensing SystemsJournal of Bacteriology, 2007
- FlhF Is Required for Swimming and Swarming in Pseudomonas aeruginosaJournal of Bacteriology, 2006
- Rhizosphere Selection of Highly Motile Phenotypic Variants of Pseudomonas fluorescens with Enhanced Competitive Colonization AbilityApplied and Environmental Microbiology, 2006
- Use of the Galleria mellonella Caterpillar as a Model Host To Study the Role of the Type III Secretion System in Pseudomonas aeruginosa PathogenesisInfection and Immunity, 2003
- Listening in on bacteria: acyl-homoserine lactone signallingNature Reviews Molecular Cell Biology, 2002
- Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectorsGene, 1985
- Studies on transformation of Escherichia coli with plasmidsJournal of Molecular Biology, 1983
- Genetic Recombination in Pseudomonas aeruginosaMicrobiology, 1955