HsfA1d, a Protein Identified via FOX Hunting Using Thellungiella salsuginea cDNAs Improves Heat Tolerance by Regulating Heat-Stress-Responsive Gene Expression
- 1 March 2013
- journal article
- Published by Elsevier BV in Molecular Plant
- Vol. 6 (2), 411-422
- https://doi.org/10.1093/mp/sst024
Abstract
Thellungiella salsuginea (formerly T. halophila), a species closely related to Arabidopsis (Arabidopsis thaliana), is tolerant not only to high salt levels, but also to chilling, freezing, and ozone. Here, we report that T. salsuginea also shows greater heat tolerance than Arabidopsis. We identified T. salsuginea HsfA1d (TsHsfA1d) as a gene that can confer marked heat tolerance on Arabidopsis. TsHsfA1d was identified via Full-length cDNA Over-eXpressing gene (FOX) hunting from among a collection of heat-stress-related T. salsuginea cDNAs. Transgenic Arabidopsis overexpressing TsHsfA1d showed constitutive up-regulation of many genes in the Arabidopsis AtHsfA1 regulon under normal growth temperature. In Arabidopsis mesophyll protoplasts, TsHsfA1d was localized in both the nucleus and the cytoplasm. TsHsfA1d also interacted with AtHSP90, which negatively regulates AtHsfA1s by forming HsfA1-HSP90 complexes in the cytoplasm. It is likely that the partial nuclear localization of TsHsfA1d induced the expression of the AtHsfA1d regulon in the transgenic plants at normal temperature. We also discovered that transgenic Arabidopsis plants overexpressing AtHsfA1d were more heat-tolerant than wild-type plants and up-regulated the expression of the HsfA1d regulon, as was observed in TsHsfA1d-overexpressing plants. We propose that the products of both TsHsfA1d and AtHsfA1d function as positive regulators of Arabidopsis heat-stress response and would be useful for the improvement of heat-stress tolerance in other plants.Keywords
Funding Information
- Ministry of Education, Culture, Sports, Science and Technology
This publication has 43 references indexed in Scilit:
- Insights into salt tolerance from the genome of Thellungiella salsugineaProceedings of the National Academy of Sciences of the United States of America, 2012
- Heat shock factors: integrators of cell stress, development and lifespanNature Reviews Molecular Cell Biology, 2010
- Nonlinear temperature effects indicate severe damages to U.S. crop yields under climate changeProceedings of the National Academy of Sciences of the United States of America, 2009
- Learning from Evolution: Thellungiella Generates New Knowledge on Essential and Critical Components of Abiotic Stress Tolerance in PlantsMolecular Plant, 2009
- Functional gene‐mining for salt‐tolerance genes with the power of ArabidopsisThe Plant Journal, 2008
- Heat stress: an overview of molecular responses in photosynthesisPhotosynthesis Research, 2008
- Galactinol and Raffinose Constitute a Novel Function to Protect Plants from Oxidative DamagePlant Physiology, 2008
- Core Genome Responses Involved in Acclimation to High TemperaturePlant Physiology, 2007
- Complexity of the heat stress response in plantsCurrent Opinion in Plant Biology, 2007
- Dual function of an Arabidopsis transcription factor DREB2A in water-stress-responsive and heat-stress-responsive gene expressionProceedings of the National Academy of Sciences of the United States of America, 2006