Cellular iron homeostasis and metabolism in plant

Abstract

Iron (Fe) homeostasis represents an important topic in the plant mineral nutrition, since Fe is an essential cofactor for fundamental biochemical activities. Due to the low availability of Fe in most soils, plants are often limited in Fe content. As a consequence, plants must tightly regulate an effective Fe acquisition, distribution, and utilization in root and leaf cells in order to allow for sustainable Fe homeostasis and metabolism. In the past several years, there has been significant progress in understanding how the Fe deficiency responses are regulated and controlled in plants. However, several questions remain still open (Vigani et al., 2013a). Thereby, further work is required in order to fully understand the Fe homeostasis and metabolism in plants. The aim of this Research Topic is to provide an overview on the new insights of both Fe homeostasis processes and metabolic reprogramming occurring in cellular compartments under different Fe nutritional conditions. Our intent was to recruit a panel of leading experts in the field of plant Fe homeostasis to prepare focused papers advancing our current knowledge in this field and contributing to the development of a comprehensive understanding of iron homeostasis in plants. Within this special issue of Frontiers, 9 original research articles, 4 reviews/mini-reviews and 2 perspective articles are collected. Four papers are based on “Omic” approaches, which in the past few years provided a huge amount of information about transcriptomic, proteomic, and metabolomic effects induced by Fe deficiency. In particular, Lan et al. (2013) investigated the role of protein phosphorylation in the regulation of cellular Fe homeostasis, by using RNA-seq analysis. The authors revealed networks comprising 87 known or annotated protein kinase and phosphatase genes that could be subdivided into several co-expressed gene modules, assigned to the leucine-rich repeat protein kinase superfamily or associated with biological processes such as “hypotonic salinity response,” “potassium ion import,” and “cellular potassium ion homeostasis.” Furthermore, Rodriguez-Celma et al. (2013), observed a dramatic down-regulation of genes belonging to photosynthesis and tetrapyrrole metabolisms by transcriptomic analysis performed on Fe-deficient Arabidopsis leaves, indicating the presence of a carefully orchestrated balance of potentially toxic tetrapyrrole intermediates and functional end products to avoid photo-oxidative damage. In addition, this study indicated six novel players with putative roles in Fe homeostasis, named Iron Responsive Proteins (IRP) 1–6. At the proteomic level, the review paper by López-Millán et al. (2013) summarized the new findings that this approach provided during the recent years. Indeed, proteomics has been a powerful tool in the elucidation of general metabolic rearrangements upon Fe deficiency. Fe deficiency has a profound impact on carbon metabolism and on the arrangement of the photosynthetic machinery, with many of these changes conserved amongst plant species. In this context, Khandakar et al. (2013) performed a small-scale proteomic approach on Hypocampus alba, confirming the main metabolic strategy of Fe-deficient plants to adjust the metabolism by an economical use of Fe and ATP. The latter including the use of energy to re-programme root morphology and function in response to Fe restriction, which should be a principal strategy for plant roots facing severely suboptimal Fe availability. Additionally they showed also that Fe deficiency affected the alkaloid biosynthesis pathways. An important topic concerning the nutrient mapping in plant tissues arises in the past recent years. About this issue, two original research papers, dealing with the Fe localization in both tissues and sub-cellular compartments have been published in the present research topic. By using Particle-Induced X-ray Emission (μPIXE) analysis, Schnell Ramos et al. (2013) determined the metal distribution in Arabidopsis seeds. μPIXE induced by a focused ion beam allows multi-elemental mapping in biological samples with high spatial resolution and high sensitivity. This fully quantitative approach revealed important Fe stores outside the endodermal cells. Imaging of imbibed seeds indicates a dynamic localization of metals as Fe and Zn concentrations increase in the subepidermal cell layer of cotyledons after imbibition. Other than quantitative approaches, also qualitative Fe localization techniques have been improved. Indeed, Roschzttardtz et al. (2013) have taken advantage of the Perls/DAB Fe staining procedure to perform a systematic analysis of Fe distribution in roots, leaves, and reproductive organs of the model plant Arabidopsis thaliana, using wild-type and mutant genotypes affected in Fe transport and storage. This study has established a reliable atlas of Fe distribution in the Arabidopsis organs, proving and refining long-assumed intracellular locations and uncovering new ones. The authors suggested that the characterization of the “iron map” in Arabidopsis will contribute to identify further actors of Fe movement in plant tissues and cell compartments. One of the Fe-dependent pathways essential to the cell is the Fe-S cluster assembly. In the present research topic Couturier et al. (2013) reviewed the new actors of this pathways that have been discovered in the past few years, such as glutaredoxin redox enzymes. Further, BolAA and NEET proteins as well as MIP18, MMS19, TAH18, DRE2 are required for the cytosolic machinery of plant Fe-S cluster biogenesis systems. It is clear that Fe-S clusters biogenesis is strictly related to a correct supply of both Fe and S. In this context Fiorieri et al. (2013) discussed that the interaction between these two nutrients might be of particular importance because Fe starvation influences S nutrition and vice...