Transient gene expression of beta-glucuronidase in citrus thin epicotyl transversal sections using particle bombardment
Open Access
- 1 January 2003
- journal article
- Published by FapUNIFESP (SciELO) in Brazilian Archives of Biology and Technology
- Vol. 46 (1), 1-6
- https://doi.org/10.1590/s1516-89132003000100001
Abstract
Studies were carried out to optimize the conditions for transient gene expression through particle bombardment on Carrizo citrange (Citrus sinensis x Poncirus trifoliata) thin epicotyl sections. The best conditions for transient GUS expression were: M-25 tungsten particles, 1550 psi helium pressure, 9 cm distance between specimen and DNA/particle holder and culture of explants in a high osmolarity medium (0.2 M mannitol + 0.2 M sorbitol) 4 h prior and 20 h after bombardment. Under these conditions, an average of 102 blue spots per bombardment (20 explants/plate) were achieved. This protocol is currently being used for transformation of Carrizo citrange and sweet orange (Citrus sinensis).Keywords
This publication has 19 references indexed in Scilit:
- In Vitro Adventitious Shoot Regeneration from Sweet Orange Using Thin Epicotyl SectionsCrop Breeding and Applied Biotechnology, 2001
- Green fluorescent protein as a screenable marker to increase the efficiency of generating transgenic woody fruit plantsTheoretical and Applied Genetics, 1999
- Agrobacterium -mediated transformation of the commercially important citrus cultivar Washington navel orangePlant Cell Reports, 1998
- Factors affecting Agrobacterium-mediated transformation in Citrus and production of sour orange (Citrus aurantium L.) plants expressing the coat protein gene of citrus tristeza virusPlant Cell Reports, 1997
- Accelerated production of transgenic wheat (Triticum aestivum L.) plantsPlant Cell Reports, 1996
- Efficient Promoter Cassettes for Enhanced Expression of Foreign Genes in Dicotyledonous and Monocotyledonous PlantsPlant and Cell Physiology, 1996
- Development and operation of five particle guns for introduction of DNA into plant cellsPlant Cell, Tissue and Organ Culture (PCTOC), 1993
- An improved assay for β-glucuronidase in transformed cells: Methanol almost completely suppresses a putative endogenous β-glucuronidase activityPlant Science, 1990
- Assaying chimeric genes in plants: The GUS gene fusion systemPlant Molecular Biology Reporter, 1987
- A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue CulturesPhysiologia Plantarum, 1962