Three-Dimensional Cell and Tissue Patterning in a Strained Fibrin Gel System
Open Access
- 21 November 2007
- journal article
- research article
- Published by Public Library of Science (PLoS) in PLOS ONE
- Vol. 2 (11), e1211
- https://doi.org/10.1371/journal.pone.0001211
Abstract
Techniques developed for the in vitro reproduction of three-dimensional (3D) biomimetic tissue will be valuable for investigating changes in cell function in tissues and for fabricating cell/matrix composites for applications in tissue engineering techniques. In this study, we show that the simple application of a continuous strain to a fibrin gel facilitates the development of fibril alignment and bundle-like structures in the fibrin gel in the direction of the applied strain. Myoblasts cultured in this gel also exhibited well-aligned cell patterning in a direction parallel to the direction of the strain. Interestingly, the direction of cell proliferation was identical to that of cell alignment. Finally, the oriented cells formed linear groups that were aligned parallel to the direction of the strain and replicated the native skeletal muscle cell patterning. In addition, vein endothelial cells formed a linear, aligned vessel-like structure in this system. Thus, the system enables the in vitro reproduction of 3D aligned cell sets replicating biological tissue patterns.Keywords
This publication has 26 references indexed in Scilit:
- Engineering tumors with 3D scaffoldsNature Methods, 2007
- Rigidity-driven growth and migration of epithelial cells on microstructured anisotropic substratesProceedings of the National Academy of Sciences of the United States of America, 2007
- Mechanical Strain Regulates Endothelial Cell PatterningIn VitroTissue Engineering, 2007
- Controlled release of nerve growth factor from fibrin gelJournal of Biomedical Materials Research Part A, 2006
- Cyclic strain increases fibroblast proliferation, matrix accumulation, and elastic modulus of fibroblast-seeded polyurethane constructsJournal of Biomechanics, 2005
- Conjugation of an antibody to cross-linked fibrin for targeted delivery of anti-restenotic drugsJournal of Controlled Release, 2004
- Inkjet printing of viable mammalian cellsBiomaterials, 2004
- Cell Shape, Cytoskeletal Tension, and RhoA Regulate Stem Cell Lineage CommitmentDevelopmental Cell, 2004
- Neuronal contact guidance in magnetically aligned fibrin gels: effect of variation in gel mechano-structural propertiesBiomaterials, 2001
- Magnetically orientated tissue-equivalent tubes: application to a circumferentially orientated media-equivalentBiomaterials, 1996