Structure and assembly of immature HIV
Open Access
- 7 July 2009
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences of the United States of America
- Vol. 106 (27), 11090-11095
- https://doi.org/10.1073/pnas.0903535106
Abstract
The major structural components of HIV are synthesized as a 55-kDa polyprotein, Gag. Particle formation is driven by the self-assembly of Gag into a curved hexameric lattice, the structure of which is poorly understood. We used cryoelectron tomography and contrast-transfer-function corrected subtomogram averaging to study the structure of the assembled immature Gag lattice to ≈17-Å resolution. Gag is arranged in the immature virus as a single, continuous, but incomplete hexameric lattice whose curvature is mediated without a requirement for pentameric defects. The resolution of the structure allows positioning of individual protein domains. High-resolution crystal structures were fitted into the reconstruction to locate protein–protein interfaces involved in Gag assembly, and to identify the structural transformations associated with virus maturation. The results of this study suggest a concept for the formation of nonsymmetrical enveloped viruses of variable sizes.Keywords
This publication has 34 references indexed in Scilit:
- Visualization of a missing link in retrovirus capsid assemblyNature, 2009
- Three-Dimensional Analysis of Budding Sites and Released Virus Suggests a Revised Model for HIV-1 MorphogenesisCell Host & Microbe, 2008
- Molecular architecture of native HIV-1 gp120 trimersNature, 2008
- Structure of B-MLV Capsid Amino-terminal Domain Reveals Key Features of Viral Tropism, Gag Assembly and Core FormationJournal of Molecular Biology, 2008
- Electron cryotomography of immature HIV-1 virions reveals the structure of the CA and SP1 Gag shellsThe EMBO Journal, 2007
- Domain-swapped dimerization of the HIV-1 capsid C-terminal domainProceedings of the National Academy of Sciences of the United States of America, 2007
- Cryo-Electron Tomographic Structure of an Immunodeficiency Virus Envelope Complex In SituPLoS Pathogens, 2006
- Retrovirus envelope protein complex structure in situ studied by cryo-electron tomographyProceedings of the National Academy of Sciences of the United States of America, 2005
- Three-dimensional Structure of HIV-1 Virus-like Particles by Electron CryotomographyJournal of Molecular Biology, 2005
- Determination of the fold of the core protein of hepatitis B virus by electron cryomicroscopyNature, 1997