Isolation of chromosomal mutations that affect carotenoid production inEscherichia coli: mutations alter copy number of ColE1-type plasmids
Open Access
- 1 February 2005
- journal article
- Published by Oxford University Press (OUP) in FEMS Microbiology Letters
- Vol. 243 (1), 227-233
- https://doi.org/10.1016/j.femsle.2004.12.015
Abstract
Chromosomal mutants were isolated in Escherichia coli that altered carotenoid production from transformed carotenoid biosynthesis genes on a pACYC-derived plasmid (pPCB15). The mutations were mapped by sequencing. One group of mutations appeared to affect the cell metabolism without changing the copy number of the carotenoid synthesis plasmid. The other group of mutations either increased or decreased the copy number of the pPCB15 plasmid as determined by real-time PCR. The copy number change in most mutants was likely specific for ColE1-type plasmids for which copy number is controlled by a small antisense RNA. This collection of host strains would be useful for fine tuning expression of proteins and adjusting production of desired molecules without recloning to different vectors.Keywords
This publication has 35 references indexed in Scilit:
- Origin-specific reduction of ColE1 plasmid copy number due to mutations in a distinct region of the Escherichia coli RNA polymeraseMolecular Genetics and Genomics, 2002
- Metabolic engineering of the terpenoid biosynthetic pathway of Escherichia coli for production of the carotenoids β-carotene and zeaxanthinBiotechnology Letters, 1999
- Amplification of λ plasmids in Escherichia coli relA mutantsJournal of Biotechnology, 1995
- Differential Replication of Plasmids during Stringent and Relaxed Response of Escherichia coliPlasmid, 1994
- High copy number of the pUC plasmid results from a Rom/Rop‐suppressible point mutation in RNA IIMolecular Microbiology, 1992
- Mutations in a new chromosomal gene of Escherichia coli K-12, pcnB, reduce plasmid copy number of pBR322 and its derivativesMolecular Genetics and Genomics, 1986
- Control of cole 1 plasmid replication: Enhancement of binding of RNA I to the primer transcript by the rom proteinCell, 1984
- cis-acting mutations that affect rop protein control of plasmid copy number.Proceedings of the National Academy of Sciences of the United States of America, 1984
- Inhibition of ColE1 RNA primer formation by a plasmid-specified small RNA.Proceedings of the National Academy of Sciences of the United States of America, 1981
- Formation of an RNA primer for initiation of replication of ColE1 DNA by ribonuclease H.Proceedings of the National Academy of Sciences of the United States of America, 1980