Effect of Hypercholesterolemia on Myocardial Necrosis and Apoptosis in the Setting of Ischemia-Reperfusion
- 15 September 2009
- journal article
- research article
- Published by Ovid Technologies (Wolters Kluwer Health) in Circulation
- Vol. 120 (11_suppl_1), S22-30
- https://doi.org/10.1161/circulationaha.108.842724
Abstract
Background— Hypercholesterolemia is prevalent in patients who experience myocardial ischemia-reperfusion injury (IR). We investigate the impact of dietary-induced hypercholesterolemia on the myocardium in the setting of acute IR. Methods and Results— In normocholesterolemic (NC, n=7) and hypercholesterolemic (HC, n=7) Yucatan male pigs, the left anterior descending coronary artery was occluded for 60 minutes, followed by reperfusion for 120 minutes. Hemodynamic values were recorded, and TTC staining was used to assess necrosis. Oxidative stress was measured. Specific cell death and survival signaling pathways were assessed by Western blot and TUNEL staining. Infarct size was 45% greater in HC versus NC (42% versus 61%, P P =0.61). Whereas global LV function (+dP/dt, P P P P P P P P P P P Conclusions— This study demonstrates that experimental hypercholesterolemia is associated with increased myocardial oxidative stress and inflammation, attenuation of cell survival pathways, and induction of apoptosis in the ischemic territory, which together may account for the expansion of myocardial necrosis in the setting of acute IR.Keywords
This publication has 37 references indexed in Scilit:
- Rosiglitazone inhibits hypercholesterolaemia-induced myeloperoxidase upregulation--a novel mechanism for the cardioprotective effects of PPAR agonistsCardiovascular Research, 2008
- Atorvastatin Increases Myocardial Indices of Oxidative Stress in a Porcine Model of Hypercholesterolemia and Chronic IschemiaJournal of Cardiac Surgery, 2008
- The effects of therapeutic sulfide on myocardial apoptosis in response to ischemia–reperfusion injury☆☆☆European Journal of Cardio-Thoracic Surgery, 2008
- Mechanisms Underlying Acute Protection From Cardiac Ischemia-Reperfusion InjuryPhysiological Reviews, 2008
- Medium-chain Fatty Acids as Metabolic Therapy in Cardiac DiseaseCardiovascular Drugs and Therapy, 2008
- Antioxidant responses of Laeonereis acuta (Polychaeta) after exposure to hydrogen peroxideBrazilian Journal of Medical and Biological Research, 2008
- The Protective Effects of N-n-butyl Haloperidol Iodide on Myocardial Ischemia-Reperfusion Injury in Rats by Inhibiting Egr-1 OverexpressionCellular Physiology and Biochemistry, 2007
- Impaired myocardial perfusion reserve in experimental hypercholesterolemia is independent of myocardial neovascularizationAmerican Journal of Physiology-Heart and Circulatory Physiology, 2007
- Size of myocardial infarction induced by ischaemia/reperfusion is unaltered in rats with metabolic syndromeClinical Science, 2006
- Dysfunction of Endothelial Nitric Oxide Synthase and AtherosclerosisArteriosclerosis, Thrombosis, and Vascular Biology, 2004