This paper discusses the relationship between mentoring and students’ perception on science, technology, engineering, and mathematics (STEM) activities. The analysis was conducted after a series of STEM related activities called Jelajah Sains 1.0 (JS 1.0) program held by university students with secondary school students at 7 secondary schools in Johor involving 700 Form 1 students. A total of 135 science undergraduate students and lecturers volunteered as mentors. A set of questionnaire was distributed after the program to study the mentoring and students’ perception on STEM. There are 8 constructs measured in the questionnaire: mentoring (facilitation, participation, STEM and scientific knowledge) and students’ perception (tendency to choose science stream, expectation towards school science, science outside school, science in everyday life and career in STEM). The analysis indicates that facilitation has a strong relationship with positive perception among the students which suggests that the mentoring embedded in JS 1.0 has positive impact to empower STEM education among school students. Hence, JS 1.0 was continued to JS 2.0.