PS6KAmplification Characterizes a Small Subset of Anaplastic Meningiomas
Open Access
- 1 February 2001
- journal article
- Published by Oxford University Press (OUP) in American Journal of Clinical Pathology
- Vol. 115 (2), 213-218
- https://doi.org/10.1309/fvnu-7uby-dxe3-77mt
Abstract
PS6K, a putative oncogene mapped to chromosome 17q23, encodes a serine/threonine kinase, which phosphorylates ribosomal subunit 6 and is part of the insulin receptor signal transduction pathway involved in the regulation of messenger RNA translation, protein synthesis, cell cycle progression, and cell size. Comparative genomic hybridization studies have detected 17q23 amplifications in a subset of meningiomas, particularly those with aggressive histologic features. PS6K amplifications have been reported in breast cancer, another hormonally driven neoplasm. We assessed PS6K dosage in 94 archival paraffin-embedded meningiomas using dual-color fluorescence in situ hybridization. We found high-level PS6K amplifications in 3 of 22 anaplastic grade III meningiomas. Amplification was confirmed by differential polymerase chain reaction in 1 of these cases. In contrast, no amplifications were identified in 37 benign (grade I) and 35 atypical (grade II) meningiomas. To our knowledge, this represents the first report of gene amplification in primary human meningiomas. Given its exclusive association with anaplastic meningiomas, PS6K amplification likely occurs during the malignant progression of a small subset of anaplastic tumors. Further studies are needed to map the 17q23 amplicon to determine whether additional genes in this region are amplified in high-grade meningiomas.Keywords
This publication has 31 references indexed in Scilit:
- Cloning and characterization of a nuclear S6 kinase, S6 kinase-related kinase (SRK); A novel nuclear target of AktOncogene, 1999
- Characterization of S6K2, a novel kinase homologous to S6K1Oncogene, 1999
- NF2 gene mutations and allelic status of 1p, 14q and 22q in sporadic meningiomasOncogene, 1999
- Cloning and Characterization of p70S6KβDefines a Novel Family of p70 S6 KinasesBiochemical and Biophysical Research Communications, 1998
- Integrin-dependent Activation of the p70 Ribosomal S6 Kinase Signaling PathwayPublished by Elsevier BV ,1996
- Molecular genetic investigation of the neurofibromatosis type 2 tumor suppressor gene in sporadic meningiomaJournal of Neurosurgery, 1996
- Combined Molecular Genetic Studies of Chromosome 22q and the Neurofibromatosis Type 2 Gene in Central Nervous System TumorsNeurosurgery, 1995
- Detection of TP53 gene mutation in human meningiomas: A study using immunohistochemistry, polymerase chain reaction/single‐strand conformation polymorphism and dna sequencing techniques on paraffin‐embedded samplesInternational Journal of Cancer, 1995
- Molecular genetics of astrocytomas and meningiomasCurrent Opinion in Neurology, 1994
- Malignant meningioma in Gorlin's syndrome: cytogenetic and p53 gene analysisJournal of Neurosurgery, 1994