Comparative evaluation ofGiardia duodenalissequence data
- 19 June 2007
- journal article
- research article
- Published by Cambridge University Press (CUP) in Parasitology
- Vol. 134 (12), 1795-1821
- https://doi.org/10.1017/s0031182007003071
Abstract
SUMMARY: A review of theGiardia duodenalissequences currently available on the GenBank database was completed to compare the different genotyping loci (small subunit ribosomal DNA, glutamate dehydrogenase, triose-phosphate isomerase and beta giardin) for their ability to discern assemblage and subassemblage groups and infer phylogenetic relationships. In total, 405Giardia duodenalissequences were sorted and aligned to examine the substitutions within and between the assemblages – A and B (zoonotic), C and D (dogs), E (livestock), F (cats) and G (rodents). It was found that all of the genes could reproducibly group isolates into their assemblages and that the AI/AII subassemblage groups were robust and identifiable at all loci. However, the assemblage B subgroups were not reproducible at half of the loci (small subunit ribosomal DNA and beta giardin), not due to their conserved nature, but because there was insufficient sequence data of reference isolates available for comparison. It is anticipated that further investigation of these loci may reveal the core subgroups of this medically important and zoonotic assemblage and also those of others. The closer, more recent, phylogenetic relationships amongst the assemblages appear to be resolved; however, more sequence data from the current loci, and possibly new loci, will be required to establish the remaining relationships.Keywords
This publication has 77 references indexed in Scilit:
- Application of Genotyping during an Extensive Outbreak of Waterborne Giardiasis in Bergen, Norway, during Autumn and Winter 2004Applied and Environmental Microbiology, 2006
- Prevalence of Giardia duodenalis genotypes in pre-weaned dairy calvesVeterinary Parasitology, 2004
- Triosephosphate Isomerase Gene Characterization and Potential Zoonotic Transmission ofGiardia duodenalisEmerging Infectious Diseases, 2003
- Presence of human Giardia in domestic, farm and wild animals, and environmental samples suggests a zoonotic potential for giardiasisVeterinary Parasitology, 2002
- A Three Nucleotide Signature Sequence in Small Subunit rRNA Divides Human Giardia in Two Different GenotypesThe Journal of Eukaryotic Microbiology, 1995
- Unique phylogenetic position of Diplomonadida based on the complete small subunit ribosomal RNA sequence of Giardia ardeae, G. muris, G. duodenalis and Hexamita sp.The FASEB Journal, 1993
- Comparative studies on the growth dynamics of two genetically distinct isolates of Giardia duodenalis in vitroInternational Journal for Parasitology, 1992
- The cysteine-rich protein gene family of Giardia lamblia: loss of the CRP170 gene in an antigenic variant.Molecular and Cellular Biology, 1992
- Complete nucleotide sequence of the ribosomal RNA tandem repeat unit fromGiardia intestinalisNucleic Acids Research, 1990
- Phylogenetic Meaning of the Kingdom Concept: an Unusual Ribosomal RNA from Giardia lambliaScience, 1989