The assessment of cellular proliferation by immunohistochemistry: A review of currently available methods and their applications
- 1 March 1992
- journal article
- review article
- Published by Springer Science and Business Media LLC in Journal of Molecular Histology
- Vol. 24 (3), 121-131
- https://doi.org/10.1007/bf01047461
Abstract
Immunohistochemical methods using antibodies to cell cycle-related antigens may be used as a means of assessing various aspects of proliferation in tissue, and have the important advantage of preserving the spatial orientation of proliferating cells in histological sections. Currently, the most widely available antibodies for this purpose are antibodies to bromodeoxyuridine (BrdU), Ki67 and antibodies to proliferating cell nuclear antigen (PCNA). BrdU is a thymidine analogue incorporated during the S phase of the cell cycle, which can be introduced by ‘in vivo’ administration or by ‘in vitro’ incubation, and monoclonal antibodies are available to display its localization. Ki67 demonstrates a nuclear antigen expressed in all phases of the cell cycle, except G0 and early G1, but can only be applied to frozen tissue. PCNA is a nuclear antigen which is essential for DNA synthesis, two commercially available antibodies to PCNA work in paraffin-embedded tissue, but may have different staining characteristics under different conditions of fixation. The main advantages and disadvantages of these different techniques are discussed, together with their main applications to date.Keywords
This publication has 90 references indexed in Scilit:
- Nucleolar organizer regions in gastric carcinoma and its precursor stagesHistopathology, 1990
- Unraveling of mitotic control mechanismsCell, 1988
- Changes in cyclin/proliferating cell nuclear antigen distribution during DNA repair synthesis.The Journal of cell biology, 1988
- Cell Kinetic Study of Human Carcinomas Using BromodeoxyuridineCell Proliferation, 1988
- Proliferative index in breast carcinoma determined in situ by Ki67 immunostaining and its relationship to clinical and pathological variablesThe Journal of Pathology, 1987
- Simultaneous staining of exponentially growing versus plateau phase cells with the proliferation‐associated antibody Ki‐67 and propidium iodide: analysis by flow cytometryCell Proliferation, 1987
- Monoclonal antibodies to a nuclear protein (PCNA/cyclin) associated with DNA replicationExperimental Cell Research, 1987
- Increased nuclear cyclin/PCNA antigen staining of non S‐phase transformed human amnion cells engaged in nucleotide excision DNA repairFEBS Letters, 1986
- A nuclear antigen associated with cell proliferation and blast transformation.The Journal of Experimental Medicine, 1981
- The Cell CycleNew England Journal of Medicine, 1981