Neutron Reflectometry of Membrane Protein Assemblies at the Solid/Liquid Interface
- 1 January 2005
- journal article
- Published by CSIRO Publishing in Australian Journal of Chemistry
- Vol. 58 (9), 674-677
- https://doi.org/10.1071/ch05112
Abstract
Neutron reflectometry has been used to study the self-assembly of a membrane protein, OmpF, onto a gold-coated silicon substrate from solution. OmpF associates into trimers and has been modified so that each trimer binds to the gold substrate through cysteine residues. Quantitative analysis of the data revealed that 12% of the surface was covered by the oriented protein with 27600 water molecules surrounding each trimer.Keywords
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