Comparative study of the intestinal mucus barrier in normal and inflamed colon
Open Access
- 1 March 2007
- Vol. 56 (3), 343-350
- https://doi.org/10.1136/gut.2006.098160
Abstract
Aim: To study the role of mucus in the spatial separation of intestinal bacteria from mucosa. Patients and methods: Mucus barrier characteristics were evaluated using histological material obtained by biopsy from purged colon, colon prepared with enema and material from untreated appendices fixed with non-aqueous Carnoy solution. Bacteria were evaluated using fluorescence in situ hybridization, with bacterial 16S RNA probes and related to the periodic acid Schiff alcian blue stain. Biopsies from controls (n = 20), patients with self-limiting colitis (SLC; n = 20), ulcerative colitis (n = 20) and 60 randomly selected appendices were investigated. Results: The mucosal surface beneath the mucus layer was free of bacteria in ⩾80% of the normal appendices and biopsies from controls. The thickness of the mucus layer and its spread decreased with increasing severity of the inflammation; the epithelial surface showed bacterial adherence, epithelial tissue defects and deep mucosal infiltration with bacteria and leucocytes. Bacteria and leucocytes were found within mucus in all biopsy specimens from patients with ulcerative colitis, SLC, and acute appendicitis. The concentration of bacteria within mucus was inversely correlated to the numbers of leucocytes. Conclusions: The large bowel mucus layer effectively prevents contact between the highly concentrated luminal bacteria and the epithelial cells in all parts of the normal colon. Colonic inflammation is always accompanied by breaks in the mucus barrier. Although the inflammatory response gradually reduces the number of bacteria in mucus and faeces, the inflammation itself is not capable of preventing bacterial migration, adherence to and invasion of the mucosa.Keywords
This publication has 27 references indexed in Scilit:
- Microbial biofilms in the gut: visualization by electron microscopy and by acridine orange staining.2004
- Inflammatory Bowel DiseaseNew England Journal of Medicine, 2002
- Mucosal and Invading Bacteria in Patients with Inflammatory Bowel Disease Compared with ControlsScandinavian Journal of Gastroenterology, 2002
- Mucosal flora in inflammatory bowel diseaseGastroenterology, 2002
- Fusobacterium prausnitzii and Related Species Represent a Dominant Group Within the Human Fecal FloraSystematic and Applied Microbiology, 2001
- Microbial modulation of innate defense: goblet cells and the intestinal mucus layerThe American Journal of Clinical Nutrition, 2001
- The adherent gastrointestinal mucus gel layer: thickness and physical state in vivoAmerican Journal of Physiology-Gastrointestinal and Liver Physiology, 2001
- Rapid identification of Enterobacteriaceae using a novel 23S rRNA-targeted oligonucleotide probeInternational Journal of Hygiene and Environmental Health, 2000
- Mucins and mucosal protection in the gastrointestinal tract: new prospects for mucins in the pathology of gastrointestinal diseaseGut, 2000
- The intestinal mucus layer from patients with inflammatory bowel disease harbors high numbers of bacteria compared with controlsGastroenterology, 1999