Sumoylation in axons triggers retrograde transport of the RNA-binding protein La
- 31 July 2007
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences of the United States of America
- Vol. 104 (31), 12913-12918
- https://doi.org/10.1073/pnas.0611562104
Abstract
A surprisingly large population of mRNAs has been shown to localize to sensory axons, but few RNA-binding proteins have been detected in these axons. These axonal mRNAs include several potential binding targets for the La RNA chaperone protein. La is transported into axonal processes in both culture and peripheral nerve. Interestingly, La is posttranslationally modified in sensory neurons by sumoylation. In axons, small ubiquitin-like modifying polypeptides (SUMO)-La interacts with dynein, whereas native La interacts with kinesin. Lysine 41 is required for sumoylation, and sumoylation-incompetent LaK41R shows only anterograde transport, whereas WT La shows both anterograde and retrograde transport in axons. Thus, sumoylation of La determines the directionality of its transport within the axonal compartment, with SUMO-La likely recycling to the cell body.Keywords
This publication has 34 references indexed in Scilit:
- An Active Dominant Mutation of Glycyl-tRNA Synthetase Causes Neuropathy in a Charcot-Marie-Tooth 2D Mouse ModelNeuron, 2006
- mRNA Transport in Dendrites: RNA Granules, Motors, and TracksJournal of Neuroscience, 2006
- Epstein-Barr virus noncoding RNAs are confined to the nucleus, whereas their partner, the human La protein, undergoes nucleocytoplasmic shuttlingThe Journal of cell biology, 2006
- New Insights into Neuronal Regeneration: The Role of Axonal Protein Synthesis in Pathfinding and Axonal ExtensionJournal of Neurotrauma, 2006
- The La Protein-RNA Complex SurfacesMolecular Cell, 2006
- SUMOMolecular Cell, 2005
- Differential Transport and Local Translation of Cytoskeletal, Injury-Response, and Neurodegeneration Protein mRNAs in AxonsJournal of Neuroscience, 2005
- SUMO Modification of Heterogeneous Nuclear RibonucleoproteinsMolecular and Cellular Biology, 2004
- Expression of kinesin heavy chain isoforms in retinal pigment epithelial cellsCell Motility, 1995
- Shuttling of the autoantigen La between nucleus and cell surface after uv irradiation of human keratinocytesExperimental Cell Research, 1990