Monitoring of microbial souring in chemically treated, produced-water biofilm systems using molecular techniques
- 21 April 2005
- journal article
- Published by Oxford University Press (OUP) in Journal of Industrial Microbiology & Biotechnology
- Vol. 32 (4), 163-170
- https://doi.org/10.1007/s10295-005-0222-5
Abstract
The identification of bacteria in oil production facilities has previously been based on culture techniques. However, cultivation of bacteria from these often-extreme environments can lead to errors in identifying the microbial community members. In this study, molecular techniques including fluorescence in situ hybridization, PCR, denaturing gradient gel electrophoresis, and sequencing were used to track changes in bacterial biofilm populations treated with nitrate, nitrite, or nitrate + molybdate as agents for the control of sulfide production. Results indicated that nitrite and nitrate + molybdate reduced sulfide production, while nitrate alone had no effect on sulfide generation. No long-term effect on sulfide production was observed. Initial sulfate-reducing bacterial numbers were not influenced by the chemical treatments, although a significant increase in sulfate-reducing bacteria was observed after termination of the treatments. Molecular analysis showed a diverse bacterial population, but no major shifts in the population due to treatment effects were observed.Keywords
This publication has 27 references indexed in Scilit:
- Factors influencing the detection of bacterial cells using fluorescence in situ hybridization (FISH): A quantitative review of published reportsFEMS Microbiology Ecology, 2003
- Action of glutaraldehyde and nitrite against sulfate-reducing bacterial biofilmsJournal of Industrial Microbiology & Biotechnology, 2002
- Abundance and Phylogenetic Affiliation of Iron Reducers in Activated Sludge as Assessed by Fluorescence In Situ Hybridization and MicroautoradiographyApplied and Environmental Microbiology, 2002
- Phylogenetic Identification and Substrate Uptake Patterns of Sulfate-Reducing Bacteria Inhabiting an Oxic-Anoxic Sewer Biofilm Determined by Combining Microautoradiography and Fluorescent In Situ HybridizationApplied and Environmental Microbiology, 2002
- Impact of Nitrate-Mediated Microbial Control of Souring in Oil Reservoirs on the Extent of CorrosionBiotechnology Progress, 2001
- Effects of two diamine biocides on the microbial community from an oil fieldCanadian Journal of Microbiology, 1998
- Development of stable denitrifying cultures during repeated aerobic-anaerobic transient periodsWater Research, 1997
- Inductively coupled argon plasma as an ion source for mass spectrometric determination of trace elementsAnalytical Chemistry, 1980
- SPECTROPHOTOMETRIC DETERMINATION OF HYDROGEN SULFIDE IN NATURAL WATERS1Limnology and Oceanography, 1969
- The Presence of Sulphate Reducing Bacteria in Oil Field WatersScience, 1926