Increased levels of SNAP‐25 and synaptophysin in the dorsolateral prefrontal cortex in bipolar I disorder
- 15 March 2006
- journal article
- Published by Wiley in Bipolar Disorders
- Vol. 8 (2), 133-143
- https://doi.org/10.1111/j.1399-5618.2006.00300.x
Abstract
Objective: In order to identify whether the mechanisms associated with neurotransmitter release are involved in the pathologies of bipolar disorder and schizophrenia, levels of presynaptic [synaptosomal‐associated protein‐25 (SNAP‐25), syntaxin, synaptophysin, vesicle‐associated membrane protein, dynamin I] and structural (neuronal cell adhesion molecule and alpha‐synuclein) neuronal markers were measured in Brodmann's area 9 obtained postmortem from eight subjects with bipolar I disorder (BPDI), 20 with schizophrenia and 20 controls. Methods: Determinations of protein levels were carried out using Western blot techniques with specific antibodies. Levels of mRNA were measured using real‐time polymerase chain reaction. Results: In BPDI, levels of SNAP‐25 (p < 0.01) and synaptophysin (p < 0.05) increased. There were no changes in schizophrenia or any other changes in BPDI. Levels of mRNA for SNAP‐25 were decreased in BPDI (p < 0.05). Conclusion: Changes in SNAP‐25 and synaptophysin in BPDI suggest that changes in specific neuronal functions could be linked to the pathology of the disorder.Keywords
This publication has 59 references indexed in Scilit:
- The dynamin superfamily: universal membrane tubulation and fission molecules?Nature Reviews Molecular Cell Biology, 2004
- Cdk5 is essential for synaptic vesicle endocytosisNature, 2003
- Altered immunoreactivity of complexin protein in prefrontal cortex in severe mental illnessMolecular Psychiatry, 2002
- Signal transmission, rather than reception, is the underlying neurochemical abnormality in schizophreniaAustralian & New Zealand Journal of Psychiatry, 2000
- Signal Transmission, Rather Than Reception, is the Underlying Neurochemical Abnormality in SchizophreniaAustralian & New Zealand Journal of Psychiatry, 2000
- Synaptophysin gene expression in schizophreniaThe British Journal of Psychiatry, 2000
- The synaptic vesicle cycle: a cascade of protein–protein interactionsNature, 1995
- Dynamin GTPase regulated by protein kinase C phosphorylation in nerve terminalsNature, 1993
- SNAP receptors implicated in vesicle targeting and fusionNature, 1993
- Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.Proceedings of the National Academy of Sciences of the United States of America, 1979