Binding Studies on Substrate- and Enantio-Selective Molecularly Imprinted Polymers
- 1 July 1991
- journal article
- analytical biochemistry-and-clinical-analysis
- Published by Taylor & Francis Ltd in Analytical Letters
- Vol. 24 (7), 1137-1145
- https://doi.org/10.1080/00032719108052959
Abstract
A molecularly imprinted polymer was prepared using tert-butyloxycarbonyl-L-phenylalanine as the print molecule and methacrylic acid as the functional monomer. The bulk polymer obtained was ground, sieved, packed into a column and investigated in the HPLC-mode by frontal chromatography to determine the number of binding sites and dissociation constants for the enantiomers interacting with the polymer. The dissociation constant for the L-enantiomer of the print molecule was lower than for the D-enantiomer (6.3 mM and 8.1 mM, respectively). This means that the affinity for the L-enantiomer was higher than for the D-enantiomer. The number of binding sites in the polymer giving rise to these dissociation constants were determined to be 28 μmol per g dry polymer.Keywords
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