A novel cell‐adhesive scaffold material for delivering keratinocytes reduces granulation tissue in dermal wounds
- 16 January 2009
- journal article
- Published by Wiley in Wound Repair and Regeneration
- Vol. 17 (1), 127-135
- https://doi.org/10.1111/j.1524-475x.2008.00450.x
Abstract
Novel peptide‐conjugated chitosan membranes were fabricated and used to deliver keratinocytes to dermal wounds in mice. Three active peptides of 12 or 13 amino acids each, RLVSYNGIIFFLK (A5G27), ASKAIQVFLLAG (A5G33), and AGTFALRGDNPQG (A99) were selected from a cell‐adhesive peptide library of laminin, a major constituent of basement membrane. The peptides were synthesized and coupled to chitosan membranes, and the resulting peptide–chitosan membranes were tested for keratinocyte attachment. Two of the peptides that bind to cell surface heparin‐like receptors (A5G27 and A5G33) were found to promote strong keratinocyte attachment, whereas the one that binds to integrin (A99) was inactive. Subsequently, A5G27– and A5G33–chitosan membranes were tested as vehicles for keratinocyte delivery in a wound model. We found that keratinocytes were delivered into the full‐thickness wound with either membrane. Using the A5G33–chitosan membrane, we further evaluated the activity of the delivered keratinocytes in wound healing. Immunohistochemistry for granulation tissue markers, including tenascin and α‐smooth muscle actin, showed that keratinocyte delivery by the present peptide–chitosan membranes in the wound bed provided a favorable condition for keratinocyte migration along the wound surface and reduced granulation tissue formation.Keywords
This publication has 36 references indexed in Scilit:
- Keratinocyte–Fibroblast Interactions in Wound HealingJournal of Investigative Dermatology, 2007
- Spatial and Temporal Control of Laminin-332 (5) and −511 (10) Expression During Induction of Anagen Hair GrowthJournal of Histochemistry & Cytochemistry, 2006
- A simplified laminin nomenclatureMatrix Biology, 2005
- Cell adhesion ability of artificial extracellular matrix proteins containing a long repetitive Arg-Gly-Asp sequenceJournal of Bioscience and Bioengineering, 2005
- Perspective Article: Tissue repair, contraction, and the myofibroblastWound Repair and Regeneration, 2005
- Immobilization of Arg-Gly-Asp (RGD) sequence in a thermosensitive hydrogel for cell delivery using pheochromocytoma cells (PC12)Journal of Bioscience and Bioengineering, 2004
- Laminin‐1 peptide‐conjugated chitosan membranes as a novel approach for cell engineeringThe FASEB Journal, 2003
- Integrins: Bidirectional, Allosteric Signaling MachinesCell, 2002
- Identification of Cell Binding Sites in the Laminin α5-Chain G DomainExperimental Cell Research, 2002
- Established mouse liver cell lines as a model system for studying epithelio-mesenchymal interactions in morphogenesisCell Differentiation and Development, 1990