Identification of a Gene Cluster EnablingLactobacillus caseiBL23 To Utilizemyo-Inositol
Open Access
- 15 June 2007
- journal article
- research article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 73 (12), 3850-3858
- https://doi.org/10.1128/aem.00243-07
Abstract
Genome analysis of Lactobacillus casei BL23 revealed that, compared to L. casei ATCC 334, it carries a 12.8-kb DNA insertion containing genes involved in the catabolism of the cyclic polyol myo-inositol (MI). Indeed, L. casei ATCC 334 does not ferment MI, whereas strain BL23 is able to utilize this carbon source. The inserted DNA consists of an iolR gene encoding a DeoR family transcriptional repressor and a divergently transcribed iolTABCDG1G2EJK operon, encoding a complete MI catabolic pathway, in which the iolK gene probably codes for a malonate semialdehyde decarboxylase. The presence of iolK suggests that L. casei has two alternative pathways for the metabolism of malonic semialdehyde: (i) the classical MI catabolic pathway in which IolA (malonate semialdehyde dehydrogenase) catalyzes the formation of acetyl-coenzyme A from malonic semialdehyde and (ii) the conversion of malonic semialdehyde to acetaldehyde catalyzed by the product of iolK. The function of the iol genes was verified by the disruption of iolA, iolT, and iolD, which provided MI-negative strains. By contrast, the disruption of iolK resulted in a strain with no obvious defect in MI utilization. Transcriptional analyses conducted with different mutant strains showed that the iolTABCDG1G2EJK cluster is regulated by substrate-specific induction mediated by the inactivation of the transcriptional repressor IolR and by carbon catabolite repression mediated by the catabolite control protein A (CcpA). This is the first example of an operon for MI utilization in lactic acid bacteria and illustrates the versatility of carbohydrate utilization in L. casei BL23.Keywords
This publication has 50 references indexed in Scilit:
- Identification of a Functional 2-keto-myo-Inositol Dehydratase Gene ofSinorhizobium frediiUSDA191 Required formyo-Inositol UtilizationBioscience, Biotechnology, and Biochemistry, 2006
- How Phosphotransferase System-Related Protein Phosphorylation Regulates Carbohydrate Metabolism in BacteriaMicrobiology and Molecular Biology Reviews, 2006
- Characterization of myo -Inositol Utilization by Corynebacterium glutamicum : the Stimulon, Identification of Transporters, and Influence on l -Lysine FormationJournal of Bacteriology, 2006
- Comparative genomics of the lactic acid bacteriaProceedings of the National Academy of Sciences of the United States of America, 2006
- -Web, an online tool to assess composition similarity of individual nucleic acid sequencesBioinformatics, 2005
- Investigation of myo-Inositol Catabolism in Rhizobium leguminosarum bv. viciae and Its Effect on Nodulation CompetitivenessMolecular Plant-Microbe Interactions®, 2001
- Interaction of a Repressor and its Binding Sites for Regulation of the Bacillussubtilis iol DivergonJournal of Molecular Biology, 1999
- Gapped BLAST and PSI-BLAST: a new generation of protein database search programsNucleic Acids Research, 1997
- CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choiceNucleic Acids Research, 1994
- Basic local alignment search toolJournal of Molecular Biology, 1990