Structure and expression properties of the endo-β-1,4-glucanase A gene from the filamentous fungusAspergillus nidulans
Open Access
- 1 June 1999
- journal article
- Published by Oxford University Press (OUP) in FEMS Microbiology Letters
- Vol. 175 (2), 239-245
- https://doi.org/10.1111/j.1574-6968.1999.tb13626.x
Abstract
Endo-β-1,4-glucanase A (EG A) of Aspergillus nidulans was purified to homogeneity, and its genomic gene (eglA) was cloned based on partial amino acid sequences of the purified enzyme and sequenced. The eglA gene comprised 1228 bp with four putative introns and encoded a polypeptide of 326 amino acids bearing high homology to the family A cellulases. The eglA promoter activity in A. nidulans was examined using the A. oryzae Taka-amylase A gene as a reporter. Expression of the reporter gene was induced by carboxymethylcellulose and cellobiose, and repressed by glucose, galactose, mannose, xylose, sorbitol, glycerol and succinate. Lactose neither induced nor repressed the expression.Keywords
This publication has 18 references indexed in Scilit:
- An Aspergillus nidulans nuclear protein, AnCP, involved in enhancement of Taka-amylase A gene expression, binds to the CCAAT-containing taaG2, amdS, and gatA promotersMolecular Genetics and Genomics, 1997
- New families in the classification of glycosyl hydrolases based on amino acid sequence similaritiesBiochemical Journal, 1993
- Characterization of the Trichoderma reesei cbh2 promoterCurrent Genetics, 1993
- Role of the two-component leader sequence and mature amino acid sequences in extracellular export of endoglucanase EGL from Pseudomonas solanacearumJournal of Bacteriology, 1992
- A classification of glycosyl hydrolases based on amino acid sequence similaritiesBiochemical Journal, 1991
- Isolation of a cDNA encoding Aspergillus oryzae Taka-amylase A: evidence for multiple related genesGene, 1989
- Development of a high-frequency transforming vector for Aspergillus nidulansGene, 1985
- Organization and Expression of Eucaryotic Split Genes Coding for ProteinsAnnual Review of Biochemistry, 1981
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970
- A PHOTOMETRIC ADAPTATION OF THE SOMOGYI METHOD FOR THE DETERMINATION OF GLUCOSEJournal of Biological Chemistry, 1944